Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies

  • Authors:
    • E. De Carlo
    • S. Baiguera
    • M. T. Conconi
    • S. Vigolo
    • C. Grandi
    • S. Lora
    • C. Martini
    • P. Maffei
    • G. Tamagno
    • R. Vettor
    • N. Sicolo
    • P. P. Parnigotto
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/ijmm_00000330
  • Pages: 195-202
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Abstract

Increasing pancreatic islet survival and function is a starting point for obtaining a valuable bioartificial pancreas for the treatment of type 1 diabetes. In this context, decellularized matrices, obtained after the removal of tissue cellular part, are known to support in vitro adhesion, growth, and function of several cell types. We demonstrate that a homologous acellular pancreatic matrix is a suitable scaffold for rat islet cultures maintaining their long-term viability and function. Islets adhered to the pancreatic matrix showed a constant glucose-induced insulin release during long-term in vitro incubation, while islets cultured without a matrix or on the liver matrix showed a progressive reduction. In order to obtain implantable devices, acellular matrix/islet cultures were entrapped into poly(vinyl alcohol) (PVA)/ poly(ethylene glycol) (PEG) tubes obtained by the freezing/thawing procedure. Under this condition, an in vitro constant insulin release was detected. The devices were then implanted into diabetic rats where reduced insulin requirement was noted suggesting insulin secretory activity of islets contained in the device. Indeed, immunofluorescence confirmed the presence of insulin- and glucagon-producing cells into the explanted devices. These data show that PVA/PEG semi-permeable membrane can obtain devices that restore, at least in part, insulin secretion.

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February 2010
Volume 25 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
De Carlo E, Baiguera S, Conconi MT, Vigolo S, Grandi C, Lora S, Martini C, Maffei P, Tamagno G, Vettor R, Vettor R, et al: Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies. Int J Mol Med 25: 195-202, 2010
APA
De Carlo, E., Baiguera, S., Conconi, M.T., Vigolo, S., Grandi, C., Lora, S. ... Parnigotto, P.P. (2010). Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies. International Journal of Molecular Medicine, 25, 195-202. https://doi.org/10.3892/ijmm_00000330
MLA
De Carlo, E., Baiguera, S., Conconi, M. T., Vigolo, S., Grandi, C., Lora, S., Martini, C., Maffei, P., Tamagno, G., Vettor, R., Sicolo, N., Parnigotto, P. P."Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies". International Journal of Molecular Medicine 25.2 (2010): 195-202.
Chicago
De Carlo, E., Baiguera, S., Conconi, M. T., Vigolo, S., Grandi, C., Lora, S., Martini, C., Maffei, P., Tamagno, G., Vettor, R., Sicolo, N., Parnigotto, P. P."Pancreatic acellular matrix supports islet survival and function in a synthetic tubular device: In vitro and in vivo studies". International Journal of Molecular Medicine 25, no. 2 (2010): 195-202. https://doi.org/10.3892/ijmm_00000330