The anti-cancer efficacy of curcumin scrutinized through core signaling pathways in glioblastoma

  • Authors:
    • Chin-Cheng Su
    • Mei-Jen Wang
    • Tsung-Lang Chiu
  • View Affiliations

  • Published online on: August 1, 2010     https://doi.org/10.3892/ijmm_00000455
  • Pages:217-224
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Abstract

Curcumin has been verified as an anti-cancer compound via multiple molecular targets. Its effective mechanisms include cell cycle arrest, inducing apoptosis, suppressing oncogenes, and enhancing tumor suppressor genes. The resistance of cells to chemotherapy, however, derives from the variable genetic aberration of cancer cells. Consequently, the core signaling pathways of glioblastoma have been explored to evaluate the efficacy of curcumin in proceeding through mutated genes in those pathways. In this study, the efficacy of curcumin was investigated in DBTRG cells. The cytotoxic ability was detected with MTT assay, and the influence of the cell cycle was checked with flow cytometry. The influence of the core signaling pathways was evaluated by Western blotting through the predominantly mutated proteins which included p53, p21, and cdc2 in the p53 pathway, CDKN2A/p16 and RB in the RB pathway, and EGFR, mTOR, Ras, PTEN, and Akt in the RTK-Ras-PI3K pathway. In addition, the apoptotic effect was determined by apoptosis-associated proteins Bcl-2, Bax, and caspase 3. Curcumin exhibits superior cytotoxicity on glioblastoma in a dose- and time-dependent manner in the MTT assay. In the core signaling pathways of glioblastoma, curcumin either significantly influences the p53 pathway by enhancing p53 and p21 and suppressing cdc2 or significantly inhibits the RB pathway by enhancing CDKN2A/p16 and suppressing phosphorylated RB. In the apoptotic pathway, the Bax and caspase 3 are significantly suppressed by curcumin and the Giemsa stain elucidates apoptotic features of DBTRG cells as well. In conclusion, curcumin appears to be an effective anti-glioblastoma drug through inhibition of the two core signaling pathways and promotion of the apoptotic pathway.

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August 2010
Volume 26 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Su, C., Wang, M., & Chiu, T. (2010). The anti-cancer efficacy of curcumin scrutinized through core signaling pathways in glioblastoma . International Journal of Molecular Medicine, 26, 217-224. https://doi.org/10.3892/ijmm_00000455
MLA
Su, C., Wang, M., Chiu, T."The anti-cancer efficacy of curcumin scrutinized through core signaling pathways in glioblastoma ". International Journal of Molecular Medicine 26.2 (2010): 217-224.
Chicago
Su, C., Wang, M., Chiu, T."The anti-cancer efficacy of curcumin scrutinized through core signaling pathways in glioblastoma ". International Journal of Molecular Medicine 26, no. 2 (2010): 217-224. https://doi.org/10.3892/ijmm_00000455