TAT-Hsp27 promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells through β1 integrin-mediated signaling

  • Authors:
    • Jong-Hwan Park
    • Ji-Hye Yoon
    • Young-Sin Lim
    • Ho-Keel Hwang
    • Soo-A Kim
    • Sang-Gun Ahn
    • Jung-Hoon Yoon
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/ijmm_00000475
  • Pages: 373-378
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Abstract

Odontoblasts are involved in tooth repair and regeneration as well as dentin formation. The aim of this study was to examine whether delivery of heat shock protein 27 (Hsp27) into cells using a TAT fusion protein system (TAT-Hsp27) enhances adhesion and migration of murine dental papilla-derived MDPC-23 cells. Hsp27 was delivered into cells by the TAT-fusion protein system. To examine whether TAT-Hsp27 affects the viability of MDPC-23 cells, MTT assay was performed. The effect of TAT-Hsp27 on adhesion and migration of MDPC-23 cells was determined using type I collagen-coated plates and a commercial kit, respectively. In addition, a precise molecular mechanism was examined by Western blot analysis and focal adhesion activity. TAT-fusion protein system delivered Hsp27 into cells successfully. Transduction of TAT-Hsp27 induced adhesion and migration of MDPC-23 cells in a dose-dependent manner. Moreover, transduction of TAT-Hsp27 increased the protein expression of β1 integrin and focal adhesion formation, and induced phosphorylation of FAK and ERK. TAT-Hsp27-induced migration of MDPC-23 cells was restored by treatment of anti-β1 integrin antibody. These findings suggest that TAT-Hsp27 promotes adhesion and migration of MDPC-23 cells via β1 integrin-mediated signaling and is a promising candidate for therapeutic application of dental pulp regeneration.

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September 2010
Volume 26 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park J, Yoon J, Lim Y, Hwang H, Kim S, Ahn S and Yoon J: TAT-Hsp27 promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells through β1 integrin-mediated signaling . Int J Mol Med 26: 373-378, 2010
APA
Park, J., Yoon, J., Lim, Y., Hwang, H., Kim, S., Ahn, S., & Yoon, J. (2010). TAT-Hsp27 promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells through β1 integrin-mediated signaling . International Journal of Molecular Medicine, 26, 373-378. https://doi.org/10.3892/ijmm_00000475
MLA
Park, J., Yoon, J., Lim, Y., Hwang, H., Kim, S., Ahn, S., Yoon, J."TAT-Hsp27 promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells through β1 integrin-mediated signaling ". International Journal of Molecular Medicine 26.3 (2010): 373-378.
Chicago
Park, J., Yoon, J., Lim, Y., Hwang, H., Kim, S., Ahn, S., Yoon, J."TAT-Hsp27 promotes adhesion and migration of murine dental papilla-derived MDPC-23 cells through β1 integrin-mediated signaling ". International Journal of Molecular Medicine 26, no. 3 (2010): 373-378. https://doi.org/10.3892/ijmm_00000475