Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury

  • Authors:
    • Sang-Wan Seo
    • Gi-Sang Bae
    • Sung Gyu Kim
    • Seung-Won Yun
    • Min-Sun Kim
    • Ki-Jung Yun
    • Rae-Kil Park
    • Ho-Joon Song
    • Sung-Joo Park
  • View Affiliations

  • Published online on: November 8, 2010     https://doi.org/10.3892/ijmm.2010.548
  • Pages: 53-61
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Curcuma longa (CL) has been reported to possess a variety of pharmacological activities. However, the effects of CL on acute pancreatitis (AP) have not yet been determined. To this end, we examined the effects of CL on cerulein-induced AP. Cell viability and cytokine productions were measured in pancreatic acini. Mice were divided into 3 groups: i) Normal group, ii) normal saline-treated group, iii) group treated with CL at a dose of 0.05, 0.1, 0.5 and 1 g/kg. CL was administered orally to mice for 7 days. The mice were intraperitoneally injected with the stable cholecystokinin analogue, cerulein (50 μg/kg), every hour for a total of 6 h. The mice were sacrificed 6 h after the completion of the cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphological examination, measurement of tissue myeloperoxidase activity, as well as the level of cytokines and heme oxygenase-1 (HO-1). The CL treatment reduced cerulein-induced cell death and cytokine production in pancreatic acini. The administration of CL significantly ameliorated the severity of pancreatitis and pancreatitis-associated lung injury, as was shown by the reduction in pancreatic edema, neutrophil infiltration, vacuolization, necrosis, serum amylase, lipase and cytokine levels, and mRNA expression of multiple inflammatory mediators such as interleukin (IL)-1ß and -6 and tumor necrosis factor (TNF)-α. In order to identify the regulatory mechanism of CL on cerulein-induced pancreatitis, we examined the level of HO-1 in the pancreas. We found that the administration of CL induced HO-1. Our results suggest that CL plays a protective role in the development of AP and pancreatitis-associated lung injury.

Related Articles

Journal Cover

January 2011
Volume 27 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Seo S, Bae G, Kim SG, Yun S, Kim M, Yun K, Park R, Song H and Park S: Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury. Int J Mol Med 27: 53-61, 2011.
APA
Seo, S., Bae, G., Kim, S.G., Yun, S., Kim, M., Yun, K. ... Park, S. (2011). Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury. International Journal of Molecular Medicine, 27, 53-61. https://doi.org/10.3892/ijmm.2010.548
MLA
Seo, S., Bae, G., Kim, S. G., Yun, S., Kim, M., Yun, K., Park, R., Song, H., Park, S."Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury". International Journal of Molecular Medicine 27.1 (2011): 53-61.
Chicago
Seo, S., Bae, G., Kim, S. G., Yun, S., Kim, M., Yun, K., Park, R., Song, H., Park, S."Protective effects of Curcuma longa against cerulein-induced acute pancreatitis and pancreatitis-associated lung injury". International Journal of Molecular Medicine 27, no. 1 (2011): 53-61. https://doi.org/10.3892/ijmm.2010.548