Group B Streptococcus serotypes III and V induce apoptosis and necrosis of human epithelial A549 cells

  • Authors:
    • Andréia Ferreira Eduardo Da Costa
    • Camila Serva Pereira
    • Gabriela Da Silva Santos
    • Técia Maria Ulisses Carvalho
    • Raphael Hirata
    • Ana Luiza De Mattos-Guaraldi
    • Ana Cláudia De Paula Rosa
    • Prescilla Emy Nagao
  • View Affiliations

  • Published online on: March 2, 2011     https://doi.org/10.3892/ijmm.2011.635
  • Pages:739-744
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Abstract

Although group B Streptococcus (GBS) has been classically described as an exclusively extracellular pathogen, growing evidence suggests that it may be internalized by epithelial cells. However, the fates of intracellular GBS and of infected respiratory epithelial cells remain unclear. Little is known about the bacterial components involved in these processes. The present study investigated the bacterial internalization by A549 cells and the apoptosis/necrosis of the infected human epithelial cells. The morphological changes in A549 cells observed from 2 h post-infection with GBS included vacuolization and the formation of apoptotic bodies. Flow cytometry revealed that 81.2% of apoptotic A549 cells were infected with GBS serotype III 90356-liquor. Moreover, a double-staining assay using propidium iodide (PI)/Annexin V (AV) gave information about the numbers of viable (PI-/AV-) (18.27%) vs. early apoptotic (PI-/AV+) (73.83%) and late apoptotic cells (PI+/AV+) (7.37%) during infection of A549 cells with GBS III 90356-liquor. In addition, 37% necrotic cells were observed in A549 cells infected with GBS serotype V 90186-blood. In conclusion, GBS serotypes III and V induce apoptosis of epithelial cells in the early stages of GBS infection, resulting in tissue destruction, bacterial spreading and, in consequence, invasive disease or systemic infection.

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May 2011
Volume 27 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Da Costa, A.F., Pereira, C.S., Da Silva Santos, G., Carvalho, T.M., Hirata, R., De Mattos-Guaraldi, A.L. ... Nagao, P.E. (2011). Group B Streptococcus serotypes III and V induce apoptosis and necrosis of human epithelial A549 cells. International Journal of Molecular Medicine, 27, 739-744. https://doi.org/10.3892/ijmm.2011.635
MLA
Da Costa, A. F., Pereira, C. S., Da Silva Santos, G., Carvalho, T. M., Hirata, R., De Mattos-Guaraldi, A. L., De Paula Rosa, A. C., Nagao, P. E."Group B Streptococcus serotypes III and V induce apoptosis and necrosis of human epithelial A549 cells". International Journal of Molecular Medicine 27.5 (2011): 739-744.
Chicago
Da Costa, A. F., Pereira, C. S., Da Silva Santos, G., Carvalho, T. M., Hirata, R., De Mattos-Guaraldi, A. L., De Paula Rosa, A. C., Nagao, P. E."Group B Streptococcus serotypes III and V induce apoptosis and necrosis of human epithelial A549 cells". International Journal of Molecular Medicine 27, no. 5 (2011): 739-744. https://doi.org/10.3892/ijmm.2011.635