Curcumin stimulates proliferation, stemness acting signals and migration of 3T3-L1 preadipocytes

  • Authors:
    • Jeong-Hwan Kim
    • Shin-Hyung Park
    • Soo-Wan Nam
    • Hyun-Joo Kwon
    • Byung-Woo Kim
    • Wun-Jae Kim
    • Yung Hyun Choi
  • View Affiliations

  • Published online on: April 20, 2011     https://doi.org/10.3892/ijmm.2011.680
  • Pages: 429-435
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In the present study, the potential of curcumin to stimulate proliferation, stemness acting signals and migration of 3T3-L1 preadipocytes and the associated molecular mechanisms were investigated. Low concentrations of curcumin stimulated cell proliferation, whereas high concentrations were cytotoxic to 3T3-L1 cells. In particular, application of 0.02 µM of curcumin for 24 h resulted in significantly increased cell proliferation and was determined to be the optimal treatment for this study. In a colony-forming cell assay, cells treated with 0.02 µM of curcumin showed an approximately 1.5-fold increase in colony formation. Curcumin treatment up-regulated the proliferation-related marker proteins coupled with increased cell growth, telomerase activity and overexpression of stemness acting signals, which was associated with activation of the phosphoinositide 3-kinase (PI3K) pathway. In addition, curcumin significantly inactivated p38 mitogen-activated protein kinases (MAPK) and stress-activated protein kinase/c-Jun N-terminal kinases (SARK/JNK), coupled with inhibition of p53 and p21 tumor suppressor gene products. In addition, curcumin significantly increased cell migration through activation of migration-associated transcription factors. Therefore, these results clearly show that activation of cell proliferation by curcumin is associated with improved stem cell potency in 3T3-L1 preadipocytes.

Related Articles

Journal Cover

September 2011
Volume 28 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Park S, Nam S, Kwon H, Kim B, Kim W and Choi YH: Curcumin stimulates proliferation, stemness acting signals and migration of 3T3-L1 preadipocytes. Int J Mol Med 28: 429-435, 2011.
APA
Kim, J., Park, S., Nam, S., Kwon, H., Kim, B., Kim, W., & Choi, Y.H. (2011). Curcumin stimulates proliferation, stemness acting signals and migration of 3T3-L1 preadipocytes. International Journal of Molecular Medicine, 28, 429-435. https://doi.org/10.3892/ijmm.2011.680
MLA
Kim, J., Park, S., Nam, S., Kwon, H., Kim, B., Kim, W., Choi, Y. H."Curcumin stimulates proliferation, stemness acting signals and migration of 3T3-L1 preadipocytes". International Journal of Molecular Medicine 28.3 (2011): 429-435.
Chicago
Kim, J., Park, S., Nam, S., Kwon, H., Kim, B., Kim, W., Choi, Y. H."Curcumin stimulates proliferation, stemness acting signals and migration of 3T3-L1 preadipocytes". International Journal of Molecular Medicine 28, no. 3 (2011): 429-435. https://doi.org/10.3892/ijmm.2011.680