Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability of the MHCC97-H human hepatocellular carcinoma cell line

  • Authors:
    • Jian Ruan
    • Feiye Liu
    • Xiaohua Chen
    • Peng Zhao
    • Ning Su
    • Guozhu Xie
    • Jianpeng Chen
    • Dayong Zheng
    • Rongcheng Luo
  • View Affiliations

  • Published online on: May 20, 2011     https://doi.org/10.3892/ijmm.2011.704
  • Pages: 497-503
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Abstract

Glypican-3 (GPC3), a membrane-bound heparan sulfate proteoglycan, is found to be overexpressed in hepatocellular carcinoma (HCC). The purpose of the present study was to investigate the possible role of GPC3 in the development of HCC. In this study, RNA interference (RNAi) with a GPC3 small hairpin RNA (GPC3 shRNA) was used to identify the effects of GPC3 on the regulation of malignant behaviors of HCC. MHCC97-H, a highly metastatic human HCC cell line in which GPC3 mRNA and protein levels were detected as the highest among the 4 HCC cell lines assessed in this study, and was thus selected as a cell model for in vitro and in vivo experiments. The results showed that down-regulation of GPC3 can significantly inhibit the proliferative and invasive ability of MHCC97-H. Compared with the parental HCC cells, GPC3-silenced cells exhibited attenuated capacities in developing tumors in nude mice, while the growth of tumor xenografts derived from these cells dramatically regressed. In conclusion, our results suggest that GPC3 contributes to the proliferation and metastasis of HCC and that it may be a potential molecular target for HCC treatment.

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October 2011
Volume 28 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Ruan J, Liu F, Chen X, Zhao P, Su N, Xie G, Chen J, Zheng D and Luo R: Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability of the MHCC97-H human hepatocellular carcinoma cell line. Int J Mol Med 28: 497-503, 2011
APA
Ruan, J., Liu, F., Chen, X., Zhao, P., Su, N., Xie, G. ... Luo, R. (2011). Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability of the MHCC97-H human hepatocellular carcinoma cell line. International Journal of Molecular Medicine, 28, 497-503. https://doi.org/10.3892/ijmm.2011.704
MLA
Ruan, J., Liu, F., Chen, X., Zhao, P., Su, N., Xie, G., Chen, J., Zheng, D., Luo, R."Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability of the MHCC97-H human hepatocellular carcinoma cell line". International Journal of Molecular Medicine 28.4 (2011): 497-503.
Chicago
Ruan, J., Liu, F., Chen, X., Zhao, P., Su, N., Xie, G., Chen, J., Zheng, D., Luo, R."Inhibition of glypican-3 expression via RNA interference influences the growth and invasive ability of the MHCC97-H human hepatocellular carcinoma cell line". International Journal of Molecular Medicine 28, no. 4 (2011): 497-503. https://doi.org/10.3892/ijmm.2011.704