Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis

  • Authors:
    • Tatsuhiko Kutsuna
    • Hirofumi Inoue
    • Haruhiko Takeda
    • Toshiaki Takahashi
    • Haruyasu Yamamoto
    • Hiromasa Miura
    • Shigeki Higashiyama
  • View Affiliations

  • Published online on: August 5, 2011     https://doi.org/10.3892/ijmm.2011.766
  • Pages: 829-834
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Abstract

Transforming growth factor (TGF)-β and bone morphogenetic protein (BMP) induce a cartilage-specific extracellular matrix (ECM) gene, aggrecan, in a chondrogenic cell line, ATDC5. The results of our recent study show that TGF-β1, but not BMP-4, strongly induces an ECM gene, fibronectin, during chondrogenesis. However, the role of fibronectin in chondrogenesis is unclear. In the current study, our results showed that TGF-β1, but not BMP-4, led to versican-dominant proteoglycan production during chondrogenesis of ATDC5 cells. siRNA-mediated reduction of fibronectin and interference in the liaison between fibronectin and integrins by the Arg-Gly-Asp-Ser (RGDS) peptide increased aggrecan expression, and decreased versican expression by TGF-β1 stimulation. These data suggest that fibronectin is a critical mediator for TGF-β-specific production balance of 2 major proteoglycans, aggrecan and versican, during chondrogenesis.

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November 2011
Volume 28 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Kutsuna T, Inoue H, Takeda H, Takahashi T, Yamamoto H, Miura H and Higashiyama S: Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis. Int J Mol Med 28: 829-834, 2011.
APA
Kutsuna, T., Inoue, H., Takeda, H., Takahashi, T., Yamamoto, H., Miura, H., & Higashiyama, S. (2011). Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis. International Journal of Molecular Medicine, 28, 829-834. https://doi.org/10.3892/ijmm.2011.766
MLA
Kutsuna, T., Inoue, H., Takeda, H., Takahashi, T., Yamamoto, H., Miura, H., Higashiyama, S."Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis". International Journal of Molecular Medicine 28.5 (2011): 829-834.
Chicago
Kutsuna, T., Inoue, H., Takeda, H., Takahashi, T., Yamamoto, H., Miura, H., Higashiyama, S."Fibronectin regulates proteoglycan production balance in transforming growth factor-β1-induced chondrogenesis". International Journal of Molecular Medicine 28, no. 5 (2011): 829-834. https://doi.org/10.3892/ijmm.2011.766