microRNA expression profiling in fetal single ventricle malformation identified by deep sequencing

  • Authors:
    • Zhang-Bin Yu
    • Shu-Ping Han
    • Yun-Fei Bai
    • Chun Zhu
    • Ya Pan
    • Xi-Rong Guo
  • View Affiliations

  • Published online on: September 20, 2011     https://doi.org/10.3892/ijmm.2011.797
  • Pages: 53-60
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Abstract

microRNAs (miRNAs) have emerged as key regulators in many biological processes, particularly cardiac growth and development, although the specific miRNA expression profile associated with this process remains to be elucidated. This study aimed to characterize the cellular microRNA profile involved in the development of congenital heart malformation, through the investigation of single ventricle (SV) defects. Comprehensive miRNA profiling in human fetal SV cardiac tissue was performed by deep sequencing. Differential expression of 48 miRNAs was revealed by sequencing by oligonucleotide ligation and detection (SOLiD) analysis. Of these, 38 were down-regulated and 10 were up-regulated in differentiated SV cardiac tissue, compared to control cardiac tissue. This was confirmed by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis. Predicted target genes of the 48 differentially expressed miRNAs were analyzed by gene ontology and categorized according to cellular process, regulation of biological process and metabolic process. Pathway-Express analysis identified the WNT and mTOR signaling pathways as the most significant processes putatively affected by the differential expression of these miRNAs. The candidate genes involved in cardiac development were identified as potential targets for these differentially expressed microRNAs and the collaborative network of microRNAs and cardiac development related-mRNAs was constructed. These data provide the basis for future investigation of the mechanism of the occurrence and development of fetal SV malformations.

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January 2012
Volume 29 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yu Z, Han S, Bai Y, Zhu C, Pan Y and Guo X: microRNA expression profiling in fetal single ventricle malformation identified by deep sequencing. Int J Mol Med 29: 53-60, 2012
APA
Yu, Z., Han, S., Bai, Y., Zhu, C., Pan, Y., & Guo, X. (2012). microRNA expression profiling in fetal single ventricle malformation identified by deep sequencing. International Journal of Molecular Medicine, 29, 53-60. https://doi.org/10.3892/ijmm.2011.797
MLA
Yu, Z., Han, S., Bai, Y., Zhu, C., Pan, Y., Guo, X."microRNA expression profiling in fetal single ventricle malformation identified by deep sequencing". International Journal of Molecular Medicine 29.1 (2012): 53-60.
Chicago
Yu, Z., Han, S., Bai, Y., Zhu, C., Pan, Y., Guo, X."microRNA expression profiling in fetal single ventricle malformation identified by deep sequencing". International Journal of Molecular Medicine 29, no. 1 (2012): 53-60. https://doi.org/10.3892/ijmm.2011.797