Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion

Xia,Hongwei; Li,Mingxing; Chen,Liang; Leng,Weibing; Yuan,Dandan; Pang,Xiaohui; Chen,Liu; Li,Ronghui; Tang,Qiulin; Bi,Feng

doi:10.3892/ijmm.2013.1321

Suppression of RND3 activity by AES downregulation promotes cancer cell proliferation and invasion

Authors:
- Hongwei Xia
- Mingxing Li
- Liang Chen
- Weibing Leng
- Dandan Yuan
- Xiaohui Pang
- Liu Chen
- Ronghui Li
- Qiulin Tang
- Feng Bi
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Affiliations: Laboratory of Signal Transduction and Molecular Targeted Therapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, P.R. China, Department of Medical Oncology, West China Hospital, Sichuan University, Sichuan, Chengdu 610041, P.R. China
Published online on: March 27, 2013 https://doi.org/10.3892/ijmm.2013.1321
Pages: 1081-1086

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Abstract

Amino-terminal enhancer of split (AES) is a member of the Groucho/TLE family. Although it has no DNA-binding site, AES can regulate transcriptional activity by interacting with transcriptional factors. Emerging evidence indicates that AES may play an important role in tumor metastasis, but the molecular mechanism is still poorly understood. In this study, we found that knockdown of AES by RNA interference (RNAi) downregulated RND3 expression at the mRNA and protein levels in MDA-MB-231 and HepG2, two cancer cell lines. Furthermore, luciferase assays showed that overexpression of AES significantly enhanced RND3 promoter activity. Moreover, inhibition of AES both in MDA-MB-231 and HepG2 cells by RNAi significantly promoted cell proliferation, cell cycle progression and invasion, consistent with the effects of RNAi-mediated RND3 knockdown in these cells. For the first time, data are presented showing that alteration of the malignant behavior of cancer cells by AES is related to RND3 regulation, and these findings also provide new insights into the mechanism of AES action in regulating tumor malignancy.

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