Correlation of serum levels of complement C4a desArg with pathologically estimated severity of glomerular lesions and mesangial hypercellularity scores in patients with IgA nephropathy

Sogabe,Atsushi; Uto,Hirofumi; Kanmura,Shuji; Nosaki,Tsuyoshi; Oyamada,Miki; Tokunaga,Koki; Nishida,Chika; Fukumoto,Mayumi; Oku,Manei; Nishimoto,Kiichiro; Takenouchi,Shozo; Ido,Akio; Shimada,Yuko; Tsubouchi,Hirohito

doi:10.3892/ijmm.2013.1390

Correlation of serum levels of complement C4a desArg with pathologically estimated severity of glomerular lesions and mesangial hypercellularity scores in patients with IgA nephropathy

Authors:
- Atsushi Sogabe
- Hirofumi Uto
- Shuji Kanmura
- Tsuyoshi Nosaki
- Miki Oyamada
- Koki Tokunaga
- Chika Nishida
- Mayumi Fukumoto
- Manei Oku
- Kiichiro Nishimoto
- Shozo Takenouchi
- Akio Ido
- Yuko Shimada
- Hirohito Tsubouchi
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Affiliations: Digestive and Lifestyle Diseases, Department of Human and Environmental Sciences, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima 890-8544, Japan, Department of Internal Medicine, Nanpuh Hospital, Kagoshima 892-8512, Japan, Miyazaki Industrial Support Foundation, Sadowara, Miyazaki 880-0303, Japan
Published online on: May 24, 2013 https://doi.org/10.3892/ijmm.2013.1390
Pages: 307-314

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Abstract

The aim of the present study was to explore serum biomarkers for the pathology of IgA nephropathy using serum proteomics. The subjects were 57 patients with IgA nephropathy who were divided into two groups (group 1, n=25; group 2, n=32) and 14 healthy controls. Serum protein profiles were analyzed using the ProteinChip surface-enhanced laser desorption ionization (SELDI) system. Associations between signal intensities of proteins and histological findings in patients with IgA nephropathy were studied in group 1. Serum levels of a candidate biomarker protein (complement component C4a desArg) for IgA nephropathy were determined by enzyme linked-immunosorbent assay (ELISA) in group 2 and the relationships of these levels with histological findings were evaluated. There were significant differences in 93 protein signals between patients in group 1 and controls. Among these signals, 3 proteins at 8592, 8757 and 8806 m/z were significantly correlated with the severity of glomerular lesions. The protein at 8592 m/z was identified as C4a desArg and the signal intensity of 8592 m/z was strongly correlated with serum C4a levels, including C4a desArg, determined by ELISA. In addition, the serum levels of C4a (mainly C4a desArg) were significantly higher in patients in group 2 compared to controls and were correlated with the severity of glomerular lesions and with mesangial hypercellularity scores. In conclusion, the serum levels of complement C4a desArg are significantly higher in patients with IgA nephropathy compared to healthy controls and are significantly correlated with the severity of glomerular lesions and mesangial hypercellularity scores. Thus, serum C4a desArg is a potential biomarker for the severity of histological findings in patients with IgA nephropathy.

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