HPIP silencing inhibits TGF-β1-induced EMT in lung cancer cells

  • Authors:
    • Shaomin Shi
    • Jianjun Zhao
    • Jing Wang
    • Donghui Mi
    • Zhongsen Ma
  • View Affiliations

  • Published online on: January 5, 2017     https://doi.org/10.3892/ijmm.2017.2851
  • Pages: 479-483
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Abstract

Epithelial-mesenchymal transition (EMT) has been reported to play an important role in the migration and invasion of tumor cells. Hematopoietic pre-B-cell leukemia transcription factor (PBX)-interacting protein (HPIP/PBXIP1) has emerged as an important regulator of the development of cancer. However, the role of HPIP in lung cancer is unclear. Thus, in the present study, we investigated the role of HPIP in transforming growth factor (TGF)-β1-induced EMT in A549 lung cancer cells in vitro. Our data demonstrated that HPIP was overexpressed in the lung cancer cell lines. TGF-β1 increased the expression of HPIP in the A549 cells. In addition, HPIP silencing significantly attenuated TGF-β1-induced EMT and migration/invasion in the A549 cells. Furthermore, knockdown of HPIP greatly inhibited TGF-β1-induced phosphorylation of Smad2 in the A549 cells. In conclusion, we demonstrated that HPIP silencing suppressed TGF-β1-induced EMT in lung cancer cells by inhibiting Smad2 activation. Therefore, HPIP may be a new therapeutic target for the treatment of lung cancer.
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February-2017
Volume 39 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Shi S, Zhao J, Wang J, Mi D and Ma Z: HPIP silencing inhibits TGF-β1-induced EMT in lung cancer cells. Int J Mol Med 39: 479-483, 2017
APA
Shi, S., Zhao, J., Wang, J., Mi, D., & Ma, Z. (2017). HPIP silencing inhibits TGF-β1-induced EMT in lung cancer cells. International Journal of Molecular Medicine, 39, 479-483. https://doi.org/10.3892/ijmm.2017.2851
MLA
Shi, S., Zhao, J., Wang, J., Mi, D., Ma, Z."HPIP silencing inhibits TGF-β1-induced EMT in lung cancer cells". International Journal of Molecular Medicine 39.2 (2017): 479-483.
Chicago
Shi, S., Zhao, J., Wang, J., Mi, D., Ma, Z."HPIP silencing inhibits TGF-β1-induced EMT in lung cancer cells". International Journal of Molecular Medicine 39, no. 2 (2017): 479-483. https://doi.org/10.3892/ijmm.2017.2851