Tyrosine phosphorylation in peripheral T cells of kidney transplant recipients: analyses of baseline levels and response to T cell receptor stimulation.

  • Authors:
    • C Müller
    • M Bonmann
    • S Heidenreich
    • M G Kiehl
    • O M Koch
  • View Affiliations

  • Published online on: August 1, 1999     https://doi.org/10.3892/ijmm.4.2.141
  • Pages: 141-145
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Abstract

Impaired immunosurveillance in recipients of organ transplants has been attributed to alleviation of T cell functions. We analyzed the phosphorylation of tyrosine residues in T cells of peripheral blood, and after T cell receptor (TCR) stimulation. The TCR was stimulated by OKT3 monoclonal antibody (mAb) in non-separated heparinized blood specimens of patients (n=64) and healthy controls (n=25). After fixation and red cell lysis, lymphocytes were permeabilized by saponin. Subsequently, intracellular phosphotyrosine residues and surface CD3 antigen were stained simultaneously with specific mAbs. We analyzed transplant recipients and healthy donors for baseline levels of total cellular tyrosine phosphorylation and for increase in phosphotyrosine content following stimulation by OKT3. Phosphotyrosine levels were significantly lower in non-stimulated T cells of kidney transplant recipients compared to controls (p=0.004). There was a marked variability in the levels of tyrosine phosphorylation among transplanted patients (p=0.02). T cell receptor stimulation by OKT3 mAb in vitro led to a strong increase of tyrosine phosphorylation in all specimens of patients and healthy controls. In conclusion, we demonstrated decreased phosphotyrosine levels in T cells of kidney transplant recipients compared to healthy donors. However, increase in tyrosine phosphorylation was not impaired in all patients as a result of TCR stimulation.

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Aug 1999
Volume 4 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Müller C, Bonmann M, Heidenreich S, Kiehl M and Koch O: Tyrosine phosphorylation in peripheral T cells of kidney transplant recipients: analyses of baseline levels and response to T cell receptor stimulation.. Int J Mol Med 4: 141-145, 1999
APA
Müller, C., Bonmann, M., Heidenreich, S., Kiehl, M., & Koch, O. (1999). Tyrosine phosphorylation in peripheral T cells of kidney transplant recipients: analyses of baseline levels and response to T cell receptor stimulation.. International Journal of Molecular Medicine, 4, 141-145. https://doi.org/10.3892/ijmm.4.2.141
MLA
Müller, C., Bonmann, M., Heidenreich, S., Kiehl, M., Koch, O."Tyrosine phosphorylation in peripheral T cells of kidney transplant recipients: analyses of baseline levels and response to T cell receptor stimulation.". International Journal of Molecular Medicine 4.2 (1999): 141-145.
Chicago
Müller, C., Bonmann, M., Heidenreich, S., Kiehl, M., Koch, O."Tyrosine phosphorylation in peripheral T cells of kidney transplant recipients: analyses of baseline levels and response to T cell receptor stimulation.". International Journal of Molecular Medicine 4, no. 2 (1999): 141-145. https://doi.org/10.3892/ijmm.4.2.141