Oxidant stress regulation of IL-8 and ICAM-1 gene expression: differential activation and binding of the transcription factors AP-1 and NF-kappaB (Review).

  • Authors:
    • K A Roebuck
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  • Published online on: September 1, 1999     https://doi.org/10.3892/ijmm.4.3.223
  • Pages: 223-253
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Abstract

Reactive oxygen species (ROS), generated either extracellularly or intracellularly through ligand-receptor interactions, can function as signal transduction molecules to activate the chemotactic cytokine interleukin-8 (IL-8) and the cell surface adhesion protein, intercellular adhesion molecule-1 (ICAM-1; CD54). Together, IL-8 and ICAM-1 orchestrate the transendothelial migration of neutrophils to sites of inflammation and injury. Recent results demonstrate that oxidant stress generated directly by exogenous H2O2 differentially induce IL-8 and ICAM-1 transcription in epithelial and endothelial cells. H2O2 induces IL-8 but not ICAM-1 in the A549 type-II-like epithelial cell line, whereas in a microvessel endothelial cell line (HMEC-1) as well as in primary endothelial cells, H2O2 induces ICAM-1 but not IL-8, which is spontaneously expressed. In contrast, the pro-inflammatory cytokine TNFalpha, whose activity is dependent on the generation of intracellular ROS, induces IL-8 and ICAM-1 in both cell types. The differential induction of IL-8 and ICAM-1 by H2O2 and TNFalpha suggest that the two inflammatory stimuli target distinct redox responsive signaling pathways to activate cell type-specific gene expression. In this regard, we found that the cell type-specific pattern of IL-8 and ICAM-1 gene expression was associated with the differential activation and promoter binding of the redox regulated transcription factors AP-1 and NF-kappaB. In this review, our current understanding of the redox regulation of the IL-8 and ICAM-1 genes is summarized, and the differential roles AP-1 and NF-kappaB play in their cell type-specific expression, with particular emphasis on the differential effects induced by TNFalpha and H2O2 is discussed.

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September 1999
Volume 4 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Roebuck, K. (1999). Oxidant stress regulation of IL-8 and ICAM-1 gene expression: differential activation and binding of the transcription factors AP-1 and NF-kappaB (Review).. International Journal of Molecular Medicine, 4, 223-253. https://doi.org/10.3892/ijmm.4.3.223
MLA
Roebuck, K."Oxidant stress regulation of IL-8 and ICAM-1 gene expression: differential activation and binding of the transcription factors AP-1 and NF-kappaB (Review).". International Journal of Molecular Medicine 4.3 (1999): 223-253.
Chicago
Roebuck, K."Oxidant stress regulation of IL-8 and ICAM-1 gene expression: differential activation and binding of the transcription factors AP-1 and NF-kappaB (Review).". International Journal of Molecular Medicine 4, no. 3 (1999): 223-253. https://doi.org/10.3892/ijmm.4.3.223