Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.

  • Authors:
    • Y Hayashida
    • K Nakata
    • H Ishikawa
    • K Taniguchi
    • K Migita
    • K Nakao
    • Y Kato
    • K Eguchi
  • View Affiliations

  • Published online on: September 1, 1999     https://doi.org/10.3892/ijmm.4.3.253
  • Pages: 253-259
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Abstract

Epidermal growth factor (EGF) and heparin-binding EGF-like growth factor (HB-EGF) exert their activities through the same cell surface receptor. In the present study, the role of EGF in cell kinetics of rat gastric epithelial cell line, RGM-1, in which HB-EGF is known to function in an autocrine fashion, was investigated. EGF stimulated the mucus glycoprotein synthesis which is characterized with cell maturation, but competed with HB-EGF for cell proliferation. By flow cytometric analysis, EGF inhibited G2/M transition by 48 h after stimulation and induced apoptosis manifested by hypodiploid DNA by 72 h after stimulation. Consistent with this, DNA ladder formation together with the histone-associated DNA fragments was found in EGF-treated cells. These results indicate that EGF and HB-EGF possess different functions in RGM-1 cells and that EGF acts as a mediator of both cell maturation and apoptosis in these cells.

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Sep 1999
Volume 4 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hayashida Y, Nakata K, Ishikawa H, Taniguchi K, Migita K, Nakao K, Kato Y and Eguchi K: Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.. Int J Mol Med 4: 253-259, 1999
APA
Hayashida, Y., Nakata, K., Ishikawa, H., Taniguchi, K., Migita, K., Nakao, K. ... Eguchi, K. (1999). Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.. International Journal of Molecular Medicine, 4, 253-259. https://doi.org/10.3892/ijmm.4.3.253
MLA
Hayashida, Y., Nakata, K., Ishikawa, H., Taniguchi, K., Migita, K., Nakao, K., Kato, Y., Eguchi, K."Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.". International Journal of Molecular Medicine 4.3 (1999): 253-259.
Chicago
Hayashida, Y., Nakata, K., Ishikawa, H., Taniguchi, K., Migita, K., Nakao, K., Kato, Y., Eguchi, K."Epidermal growth factor-mediated cell maturation and apoptosis in RGM-1 rat gastric mucosal cells.". International Journal of Molecular Medicine 4, no. 3 (1999): 253-259. https://doi.org/10.3892/ijmm.4.3.253