RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling

  • Authors:
    • Jie Feng
    • Liyi Xie
    • Ranran Kong
    • Yali Zhang
    • Kehui Shi
    • Wanhong Lu
    • Hongli Jiang
  • View Affiliations

  • Published online on: September 27, 2017     https://doi.org/10.3892/ijmm.2017.3154
  • Pages:1965-1970
0

Abstract

The receptor for activated C-kinase 1 (RACK1) is a member of the WD40-repeat family of proteins and has been reported to be implicated in the development of liver fibrosis. However, the role of RACK1 in renal fibrosis remains unclear. Therefore, in this study, we investigated the effects of RACK1 on transforming growth factor-β1 (TGF-β1)-treated human proximal tubular epithelial cells and aimed to elucidate the possible mechanisms responsible for its anti-fibrotic effects. Our results revealed that RACK1 was highly expressed in the renal fibrotic tissues and TGF-β1-treated HK-2 cells. RACK1 silencing inhibited TGF-β1‑induced α-smooth muscle actin and connective tissue growth factor expression in the HK-2 cells. Furthermore, RACK1 silencing inhibited the expression of phosphorylated Smad3 in the TGF-β1-treated HK-2 cells. To the best of our knowledge, these data demonstrate for the first time the role of RACK1 in renal fibrosis. The present findings indicate that RACK1 silencing attenuates renal fibrosis by suppressing the activation of TGF-β1/Smad3 signaling pathway in HK-2 cells. Thus, RACK1 may serve as a novel regulator of renal fibrosis.

Related Articles

Journal Cover

December 2017
Volume 40 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Feng, J., Xie, L., Kong, R., Zhang, Y., Shi, K., Lu, W., & Jiang, H. (2017). RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling. International Journal of Molecular Medicine, 40, 1965-1970. https://doi.org/10.3892/ijmm.2017.3154
MLA
Feng, J., Xie, L., Kong, R., Zhang, Y., Shi, K., Lu, W., Jiang, H."RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling". International Journal of Molecular Medicine 40.6 (2017): 1965-1970.
Chicago
Feng, J., Xie, L., Kong, R., Zhang, Y., Shi, K., Lu, W., Jiang, H."RACK1 silencing attenuates renal fibrosis by inhibiting TGF-β signaling". International Journal of Molecular Medicine 40, no. 6 (2017): 1965-1970. https://doi.org/10.3892/ijmm.2017.3154