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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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Jun 2000 Volume 5 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Jun 2000 Volume 5 Issue 6

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Article

H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.

  • Authors:
    • T Watanabe
    • A Sakamoto
    • S Tamiya
    • Y Oda
    • K Masuda
    • M Tsuneyoshi
  • View Affiliations / Copyright

    Affiliations: Department of Anatomic Pathology, Pathological Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
  • Pages: 605-613
    |
    Published online on: June 1, 2000
       https://doi.org/10.3892/ijmm.5.6.605
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Abstract

It has been shown that the NF1 (neurofibromatosis type 1) gene encodes a tumor suppressor which inactivates ras proteins. Among malignant mesenchymal tumors, H-ras-1 mutations have been found in malignant fibrous histiocytoma, leiomyosarcoma and embryonal rhabdomyosarcoma. However, studies on H-ras-1 mutation of many cases of malignant peripheral nerve sheath tumors (MPNST) have not been documented. Therefore, we investigated H-ras-1 mutations of MPNST. In 45 cases of MPNSTs of our files, DNA was extracted from the formalin-fixed paraffin-embedded tissue, and the mutations of the H-ras-1 gene were detected by using PCR-RFLP (polymerase chain reaction- restriction fragment length polymorphisms) method and direct sequencing. We found two cases with H-ras-1 point mutation in MPNST for the first time. Both cases showed the same mutation in codon 13.1 [GGT(Gly) to AGT(Ser) transition]. Interestingly, both cases were associated with NF1. It is possibile that the mutation of the H-ras-1 gene occurred after the mutation of the NF1 gene in the MPNST.

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Copy and paste a formatted citation
Spandidos Publications style
Watanabe T, Sakamoto A, Tamiya S, Oda Y, Masuda K and Tsuneyoshi M: H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.. Int J Mol Med 5: 605-613, 2000.
APA
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., & Tsuneyoshi, M. (2000). H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.. International Journal of Molecular Medicine, 5, 605-613. https://doi.org/10.3892/ijmm.5.6.605
MLA
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., Tsuneyoshi, M."H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.". International Journal of Molecular Medicine 5.6 (2000): 605-613.
Chicago
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., Tsuneyoshi, M."H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.". International Journal of Molecular Medicine 5, no. 6 (2000): 605-613. https://doi.org/10.3892/ijmm.5.6.605
Copy and paste a formatted citation
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Spandidos Publications style
Watanabe T, Sakamoto A, Tamiya S, Oda Y, Masuda K and Tsuneyoshi M: H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.. Int J Mol Med 5: 605-613, 2000.
APA
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., & Tsuneyoshi, M. (2000). H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.. International Journal of Molecular Medicine, 5, 605-613. https://doi.org/10.3892/ijmm.5.6.605
MLA
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., Tsuneyoshi, M."H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.". International Journal of Molecular Medicine 5.6 (2000): 605-613.
Chicago
Watanabe, T., Sakamoto, A., Tamiya, S., Oda, Y., Masuda, K., Tsuneyoshi, M."H-ras-1 point mutation in malignant peripheral nerve sheath tumors: polymerase chain reaction restriction fragment length polymorphism analysis and direct sequencing from paraffin-embedded tissues.". International Journal of Molecular Medicine 5, no. 6 (2000): 605-613. https://doi.org/10.3892/ijmm.5.6.605
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