Human papillomavirus type 16 E7 impairs the activation of the interferon regulatory factor-1.

  • Authors:
    • S E Perea
    • P Massimi
    • L Banks
  • View Affiliations

  • Published online on: June 1, 2000     https://doi.org/10.3892/ijmm.5.6.661
  • Pages: 661-667
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Abstract

We had previously observed that HPV-16 E7 disturbs the Guanylate Binding Protein (GBP)-ISRE reporter activation by IFN-gamma thus suggesting an alteration of the IRF-1 function. In this study we examined the mechanism by which E7 affects the IFN-gamma signals driving the activation of gene transcription. Using 14/2 BRK cells containing dexamethasone-inducible HPV-16 E7 gene, we observed a large inhibition of the IRF-1 DNA binding activity upon E7 induction. Concomitantly, there was no significant change in the levels of IRF-1, indicating that this was not due to reduced levels of IRF-1 expression. Likewise, in vitro translated E7 did not affect the IRF-1 DNA binding activity in nuclear extracts derived from IFN-induced cells, thus indicating that the effects of E7 are upstream of IRF-1's binding to its DNA recognition site. Finally, NFkappaB DNA binding activity was also inhibited under conditional expression of E7. These data indicate that HPV-16 E7 inhibits the IRF-1 and NFkappaB function and this could lead to the impairment of the IFN response in HPV-infected cells. Furthermore, the findings suggest that different events of the IFN inducible signal cascade seem to be target for HPV-16 E7.

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Jun 2000
Volume 5 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Perea S, Massimi P and Banks L: Human papillomavirus type 16 E7 impairs the activation of the interferon regulatory factor-1.. Int J Mol Med 5: 661-667, 2000.
APA
Perea, S., Massimi, P., & Banks, L. (2000). Human papillomavirus type 16 E7 impairs the activation of the interferon regulatory factor-1.. International Journal of Molecular Medicine, 5, 661-667. https://doi.org/10.3892/ijmm.5.6.661
MLA
Perea, S., Massimi, P., Banks, L."Human papillomavirus type 16 E7 impairs the activation of the interferon regulatory factor-1.". International Journal of Molecular Medicine 5.6 (2000): 661-667.
Chicago
Perea, S., Massimi, P., Banks, L."Human papillomavirus type 16 E7 impairs the activation of the interferon regulatory factor-1.". International Journal of Molecular Medicine 5, no. 6 (2000): 661-667. https://doi.org/10.3892/ijmm.5.6.661