Characterization of the human peroxisome proliferator activated receptor delta gene and its expression.

  • Authors:
    • J Skogsberg
    • K Kannisto
    • L Roshani
    • E Gagne
    • A Hamsten
    • C Larsson
    • E Ehrenborg
  • View Affiliations

  • Published online on: July 1, 2000     https://doi.org/10.3892/ijmm.6.1.73
  • Pages: 73-154
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Abstract

Peroxisome proliferator activated receptors (PPARs) are nuclear receptors regulating the expression of genes involved in lipid and glucose metabolism. Three different PPARs; alpha (PPARA), gamma (PPARG) and delta (PPARD) have been characterized and they are distinguished from each other by tissue distribution and cell activation. In this study, the structure and detailed chromosomal localization of the human PPARD gene was determined. Three genomic clones containing the PPARD gene was isolated from a human P1 library. The gene spans approximately 85 kb of DNA and consists of 9 exons and 8 introns with exons ranging in size from 84 bp to 2.3 kb and introns ranging from 180 bp to 50 kb. All splice acceptor and donor sites conform to the consensus sequences including the AG-GT motif. Although PPARD lacks a TATA box, the gene is transcribed from a unique start site located 380 bp upstream of the ATG initiation codon. The 5' and 3' ends were mapped by rapid amplification of cDNA ends and the mRNA size of PPARD based upon the structure of the gene is 3803 bp. In addition, the chromosomal sublocalization of PPARD was determined by radiation hybrid mapping. The PPARD gene is located at 14 cR from the colipase gene and 15 cR from the serine kinase gene at chromosomal region 6p21.2.

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Jul 2000
Volume 6 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Skogsberg J, Kannisto K, Roshani L, Gagne E, Hamsten A, Larsson C and Ehrenborg E: Characterization of the human peroxisome proliferator activated receptor delta gene and its expression.. Int J Mol Med 6: 73-154, 2000
APA
Skogsberg, J., Kannisto, K., Roshani, L., Gagne, E., Hamsten, A., Larsson, C., & Ehrenborg, E. (2000). Characterization of the human peroxisome proliferator activated receptor delta gene and its expression.. International Journal of Molecular Medicine, 6, 73-154. https://doi.org/10.3892/ijmm.6.1.73
MLA
Skogsberg, J., Kannisto, K., Roshani, L., Gagne, E., Hamsten, A., Larsson, C., Ehrenborg, E."Characterization of the human peroxisome proliferator activated receptor delta gene and its expression.". International Journal of Molecular Medicine 6.1 (2000): 73-154.
Chicago
Skogsberg, J., Kannisto, K., Roshani, L., Gagne, E., Hamsten, A., Larsson, C., Ehrenborg, E."Characterization of the human peroxisome proliferator activated receptor delta gene and its expression.". International Journal of Molecular Medicine 6, no. 1 (2000): 73-154. https://doi.org/10.3892/ijmm.6.1.73