Growth inhibitory effect on glioma cells of adenovirus-mediated p16/INK4a gene transfer in vitro and in vivo.

  • Authors:
    • S H Lee
    • M S Kim
    • H C Kwon
    • I C Park
    • M J Park
    • C T Lee
    • Y W Kim
    • C M Kim
    • S I Hong
  • View Affiliations

  • Published online on: November 1, 2000     https://doi.org/10.3892/ijmm.6.5.559
  • Pages: 559-622
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Abstract

The tumour suppressor gene p16/INK4a encodes a specific inhibitor of the cyclin D-dependent kinases CDK4 and CDK6. p16/INK4a prevents the association of CDK4 with cyclin D1, and subsequently inhibits phosphorylation of retinoblastoma tumour suppressor protein (pRb), thus preventing exit from the G1 phase. In human cancers, the estimated frequency of genetic alteration involving the p16/INK4a locus is believed to be second only to alteration of p53. A high frequency (greater than 50%) of homozygous p16/INK4a gene deletion has been demonstrated in glioblastoma tissues and p16/INK4a is altered in 80% of glioma cell lines. Therefore, restoration of p16/INK4a would suppress cell proliferation and induce cell growth arrest. We showed here that restoration of p16/INK4a expression in p16 negative U87MG, U251MG and partially deleted U373MG by Ad-CMV-p16/INK4a induced growth suppression in vitro and in vivo. Expression of p16 transferred by Ad-CMV-p16/INK4a in glioma cells was highly efficient and maintained for more than seven days. In addition, we found that the endogenous status of p16 and Rb might affect the expression of exogenous p16/INK4a gene and inhibitory effect of cell proliferation. Even though, there were several factors affecting the efficiency of Ad-CMV-p16/INK4 gene transfer, our results suggest that Ad-CMV-p16 gene therapy strategy is potentially useful and warrants further clinical investigation for the treatment of gliomas.

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Nov 2000
Volume 6 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lee S, Kim M, Kwon H, Park I, Park M, Lee C, Kim Y, Kim C and Hong S: Growth inhibitory effect on glioma cells of adenovirus-mediated p16/INK4a gene transfer in vitro and in vivo.. Int J Mol Med 6: 559-622, 2000.
APA
Lee, S., Kim, M., Kwon, H., Park, I., Park, M., Lee, C. ... Hong, S. (2000). Growth inhibitory effect on glioma cells of adenovirus-mediated p16/INK4a gene transfer in vitro and in vivo.. International Journal of Molecular Medicine, 6, 559-622. https://doi.org/10.3892/ijmm.6.5.559
MLA
Lee, S., Kim, M., Kwon, H., Park, I., Park, M., Lee, C., Kim, Y., Kim, C., Hong, S."Growth inhibitory effect on glioma cells of adenovirus-mediated p16/INK4a gene transfer in vitro and in vivo.". International Journal of Molecular Medicine 6.5 (2000): 559-622.
Chicago
Lee, S., Kim, M., Kwon, H., Park, I., Park, M., Lee, C., Kim, Y., Kim, C., Hong, S."Growth inhibitory effect on glioma cells of adenovirus-mediated p16/INK4a gene transfer in vitro and in vivo.". International Journal of Molecular Medicine 6, no. 5 (2000): 559-622. https://doi.org/10.3892/ijmm.6.5.559