Increase in bone growth factors with healing rat fractures: The enhancing effect of zinc

  • Authors:
    • A. Igarashi
    • M. Yamaguchi
  • View Affiliations

  • Published online on: October 1, 2001     https://doi.org/10.3892/ijmm.8.4.433
  • Pages: 433-438
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The effect of zinc, a stimulator of bone formation, on bone protein components in the femoral-diaphyseal tissues with fracture healing was investigated. Rats were sacrificed between 1 and 7 days after the femoral fracture, and the diaphyseal tissues were cultured in a serum-free Dulbecco's modified Eagle's medium for 24 h. Protein content in the femoral-diaphyseal tissues was markedly elevated by fracture healing. The amount of protein in the medium cultured with the diaphyseal tissues obtained from fracture healing rats was markedly elevated as compared with that of normal rats, indicating that bone protein components were secreted into culture medium. Analysis with sodium dodecyl sulfate-polyacrylamide gel elecrophoresis (SDS-PAGE) showed that many protein molecules were secreted from the diaphyseal tissues with fracture healing. Especially, protein molecule of about 66 kDa was markedly secreted by fracture healing. The presence of zinc acexamate (10-5 and 10-4 M) in culture medium induced a significant elevation of medium protein content; the zinc effect was enhanced by culture with the diaphyseal tissues of fracture healing rats. Also, the culture of diaphyseal tissues with fracture healing caused a significant increase in insulin-like growth factor-I (IGF-I) and transforming growth factor-β1 (TGF-β1) in culture medium. The production of IGF-I and TGF-β1 from bone tissues with fracture healing was significantly enhanced in the presence of zinc acexamate (10-6-10-4 M). Moreover, the addition of IGF-I (10-8 M) or TGF-β1 (10-10 M) in a culture medium caused a significant elevation of protein content in the medium cultured with the femoral-diaphyseal tissues from normal and fracture healing rats. The effect of IGF-I or TGF-β1 was significantly enhanced in the presence of zinc acexamate (10-4 M). Also, deoxyribonucleic acid (DNA) content in the diaphyseal tissues from normal and fracture healing rats was significantly raised by the culture with IGF-I or TGF-β1. The effect of IGF-I was significantly enhanced by zinc. The present study demonstrates that fracture healing increases production of bone IGF-I and TGF-β1, and that this elevation is enhanced by zinc treatment.

Related Articles

Journal Cover

October 2001
Volume 8 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Igarashi A and Igarashi A: Increase in bone growth factors with healing rat fractures: The enhancing effect of zinc. Int J Mol Med 8: 433-438, 2001
APA
Igarashi, A., & Igarashi, A. (2001). Increase in bone growth factors with healing rat fractures: The enhancing effect of zinc. International Journal of Molecular Medicine, 8, 433-438. https://doi.org/10.3892/ijmm.8.4.433
MLA
Igarashi, A., Yamaguchi, M."Increase in bone growth factors with healing rat fractures: The enhancing effect of zinc". International Journal of Molecular Medicine 8.4 (2001): 433-438.
Chicago
Igarashi, A., Yamaguchi, M."Increase in bone growth factors with healing rat fractures: The enhancing effect of zinc". International Journal of Molecular Medicine 8, no. 4 (2001): 433-438. https://doi.org/10.3892/ijmm.8.4.433