Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR

  • Authors:
    • M. Hatta
    • H. Daitoku
    • H. Matsuzaki
    • Y. Deyama
    • Y. Yoshimura
    • K. Suzuki
    • A. Matsumoto
    • A. Fukamizu
  • View Affiliations

  • Published online on: February 1, 2002     https://doi.org/10.3892/ijmm.9.2.147
  • Pages: 147-152
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Alkaline phosphatase (ALP) is a basic marker of osteoblast maturation and osteogenesis. However, the mechanisms of the ALP gene regulation in osteoblasts remain elusive. In this study, we examined the expression of forkhead transcription factor FKHR, a regulator of hepatic glucose metabolic and proapoptotic genes, in osteogenic cells and the effect of FKHR on transcription of the ALP gene. RT-PCR and immunoblot analyses revealed the expression of FKHR in osteogenic MC3T3-E1, SaOS2, and UMR 106 cells. Reporter assays demonstrated that the overexpression of FKHR stimulated ALP promoter activity through the forkhead response element in its promoter. These results suggest that ALP is a target gene regulated by FKHR and that FKHR contributes to osteoblast maturation and osteogenesis.

Related Articles

Journal Cover

February 2002
Volume 9 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hatta M, Daitoku H, Matsuzaki H, Deyama Y, Yoshimura Y, Suzuki K, Matsumoto A and Fukamizu A: Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR. Int J Mol Med 9: 147-152, 2002
APA
Hatta, M., Daitoku, H., Matsuzaki, H., Deyama, Y., Yoshimura, Y., Suzuki, K. ... Fukamizu, A. (2002). Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR. International Journal of Molecular Medicine, 9, 147-152. https://doi.org/10.3892/ijmm.9.2.147
MLA
Hatta, M., Daitoku, H., Matsuzaki, H., Deyama, Y., Yoshimura, Y., Suzuki, K., Matsumoto, A., Fukamizu, A."Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR". International Journal of Molecular Medicine 9.2 (2002): 147-152.
Chicago
Hatta, M., Daitoku, H., Matsuzaki, H., Deyama, Y., Yoshimura, Y., Suzuki, K., Matsumoto, A., Fukamizu, A."Regulation of alkaline phosphatase promoter activity by forkhead transcription factor FKHR". International Journal of Molecular Medicine 9, no. 2 (2002): 147-152. https://doi.org/10.3892/ijmm.9.2.147