Inhibition of HIV-1 LTR-driven in vitro transcription by molecular hybrids based on peptide nucleic acids mimicking the NF-κB binding site

  • Authors:
    • Carlo Mischiati
    • Monica Borgatti
    • Giordana Feriotto
    • Cristina Rutigliano
    • Laura Breda
    • Nicoletta Bianchi
    • Roberto Gambari
  • View Affiliations

  • Published online on: June 1, 2002     https://doi.org/10.3892/ijmm.9.6.633
  • Pages: 633-639
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Abstract

The inhibition of gene transcription mediated by peptide nucleic acids (PNAs) has been described as mainly due to a process that involves strand invasion of target DNA containing homopurine stretches with generation of PNA/DNA/PNA triplexes. Scarce information is available on DNA strand invasion of mixed purine-pyrimidine stretches and theirs possible use as molecules able to interact with transcription factors. With this aim, we compared the effects of DNA/DNA, DNA/PNA or PNA/PNA hybrid molecules mimicking the NF-κB binding sites of HIV-1 on LTR-driven in vitro transcription. The results allowed us to conclude that PNA/PNA molecules and DNA/PNA hybrids are capable of inhibiting transcription, like the DNA/DNA. We investigated the mechanisms by which PNA/PNA and DNA/PNA hybrids affect the transcription. The results suggest that DNA/PNA and PNA/PNA molecules inhibit transcription in distinct manner; the inhibitory effects are due to strand invasion in the case of PNA/PNA hybrids and decoy effects on transcription factors belonging the NF-κB/Rel family in the case of DNA/PNA hybrids. It is noteworthy that DNA/PNA hybrids are more resistant to nucleases than DNA/DNA hybrids.

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June 2002
Volume 9 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Mischiati C, Borgatti M, Feriotto G, Rutigliano C, Breda L, Bianchi N and Gambari R: Inhibition of HIV-1 LTR-driven in vitro transcription by molecular hybrids based on peptide nucleic acids mimicking the NF-κB binding site. Int J Mol Med 9: 633-639, 2002
APA
Mischiati, C., Borgatti, M., Feriotto, G., Rutigliano, C., Breda, L., Bianchi, N., & Gambari, R. (2002). Inhibition of HIV-1 LTR-driven in vitro transcription by molecular hybrids based on peptide nucleic acids mimicking the NF-κB binding site. International Journal of Molecular Medicine, 9, 633-639. https://doi.org/10.3892/ijmm.9.6.633
MLA
Mischiati, C., Borgatti, M., Feriotto, G., Rutigliano, C., Breda, L., Bianchi, N., Gambari, R."Inhibition of HIV-1 LTR-driven in vitro transcription by molecular hybrids based on peptide nucleic acids mimicking the NF-κB binding site". International Journal of Molecular Medicine 9.6 (2002): 633-639.
Chicago
Mischiati, C., Borgatti, M., Feriotto, G., Rutigliano, C., Breda, L., Bianchi, N., Gambari, R."Inhibition of HIV-1 LTR-driven in vitro transcription by molecular hybrids based on peptide nucleic acids mimicking the NF-κB binding site". International Journal of Molecular Medicine 9, no. 6 (2002): 633-639. https://doi.org/10.3892/ijmm.9.6.633