PROLIFERATIVE ACTIVITY IN ADVANCED GASTRIC-CANCER WITH KI-67 AND PROPIDIUM IODIDE - ANALYSIS BY FLOW-CYTOMETRY

  • Authors:
    • H KIMURA
    • Y YONEMURA
    • I MIYAZAKI
  • View Affiliations

  • Published online on: August 1, 1992     https://doi.org/10.3892/ijo.1.3.265
  • Pages: 265-269
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Abstract

By setting the cut-off line at the level as used in a negative control study without primary antibody in the same sample, the Ki-67 labeling rate was calculated by the Ki67-DNA dual fluorescence analysis. The mean Ki-67 labeling rate of 28 advanced gastric cancer was 45.1% (13.9-76.3%). The Ki-67 labeling rates were significantly higher for larger size tumor (p<0.05), peritoneal metastasis and advanced stage (p<0.01). This is the first report that cycling cells are calculated by setting the cut-off line for solid tumors. Use of this flow cytometric procedure substantially facilitates the quantification of proliferating cells in gastric cancer.

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August 1992
Volume 1 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
KIMURA H, YONEMURA Y and MIYAZAKI I: PROLIFERATIVE ACTIVITY IN ADVANCED GASTRIC-CANCER WITH KI-67 AND PROPIDIUM IODIDE - ANALYSIS BY FLOW-CYTOMETRY. Int J Oncol 1: 265-269, 1992.
APA
KIMURA, H., YONEMURA, Y., & MIYAZAKI, I. (1992). PROLIFERATIVE ACTIVITY IN ADVANCED GASTRIC-CANCER WITH KI-67 AND PROPIDIUM IODIDE - ANALYSIS BY FLOW-CYTOMETRY. International Journal of Oncology, 1, 265-269. https://doi.org/10.3892/ijo.1.3.265
MLA
KIMURA, H., YONEMURA, Y., MIYAZAKI, I."PROLIFERATIVE ACTIVITY IN ADVANCED GASTRIC-CANCER WITH KI-67 AND PROPIDIUM IODIDE - ANALYSIS BY FLOW-CYTOMETRY". International Journal of Oncology 1.3 (1992): 265-269.
Chicago
KIMURA, H., YONEMURA, Y., MIYAZAKI, I."PROLIFERATIVE ACTIVITY IN ADVANCED GASTRIC-CANCER WITH KI-67 AND PROPIDIUM IODIDE - ANALYSIS BY FLOW-CYTOMETRY". International Journal of Oncology 1, no. 3 (1992): 265-269. https://doi.org/10.3892/ijo.1.3.265