IMMUNOGLOBULIN GENE SEQUENCE-ANALYSIS OF B-1 (CD5+B) CELL CLONES IN A MURINE MODEL OF CHRONIC LYMPHOCYTIC-LEUKEMIA AND RICHTERS SYNDROME

  • Authors:
    • F MAHBOUDI
    • JA PHILLIPS
    • ES RAVECHE
  • View Affiliations

  • Published online on: September 1, 1992     https://doi.org/10.3892/ijo.1.4.459
  • Pages: 459-465
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Abstract

The NZB mouse has recently been proposed as an animal model for the human malignancy chronic lymphocytic leukemia (CLL) because of the age-dependent onset of clonally expanded hyperdiploid B-1 cells these mice develop by one year of age. We have examined the immunoglobulin sequence of several NZB B-1 malignant clones and found these clones to have several common characteristics. The B-1 malignant clones all use unmuated VH genes and DFL16.1, a D region gene which pre-dominates during fetal B cell development. In addition, no N base insertions were observed in the clones. A continually passaged line of murine CLL-like cells was examined in more detail. This line used a germline S107 VH gene that showed no evidence of accumulated somatic mutation over the course of five years of in vivo passage. The D gene (DFL16.1) was also unmutated with no evidence of non-germline diversity at the junction sites. The non-mutated state was maintained despite a continued transformation of the CLL-like cells into a more aggressive large cell lymphoma known as Richter's syndrome. Several years after the development of Richter's syndrome, the usage of a completely new VH gene family was detected. This second B-1 clone employing a new VH gene was expressed with similar characteristics as the initial B-1 clone, both employing DFL16.1 with no N base insertions at the junction sites. This demonstrates that B-1 cells which are destined to clonally expand have unique characteristics in the expression of surface immunoglobulin. Therefore, B-1 (CD5+ B) cells which undergo transformation in CLL are not randomly derived from the normal B-1 cell population but instead come from a subpopulation of B-1 cells which display these specific features of immunoglobulin expression.

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September 1992
Volume 1 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
MAHBOUDI F, PHILLIPS J and RAVECHE E: IMMUNOGLOBULIN GENE SEQUENCE-ANALYSIS OF B-1 (CD5+B) CELL CLONES IN A MURINE MODEL OF CHRONIC LYMPHOCYTIC-LEUKEMIA AND RICHTERS SYNDROME. Int J Oncol 1: 459-465, 1992
APA
MAHBOUDI, F., PHILLIPS, J., & RAVECHE, E. (1992). IMMUNOGLOBULIN GENE SEQUENCE-ANALYSIS OF B-1 (CD5+B) CELL CLONES IN A MURINE MODEL OF CHRONIC LYMPHOCYTIC-LEUKEMIA AND RICHTERS SYNDROME. International Journal of Oncology, 1, 459-465. https://doi.org/10.3892/ijo.1.4.459
MLA
MAHBOUDI, F., PHILLIPS, J., RAVECHE, E."IMMUNOGLOBULIN GENE SEQUENCE-ANALYSIS OF B-1 (CD5+B) CELL CLONES IN A MURINE MODEL OF CHRONIC LYMPHOCYTIC-LEUKEMIA AND RICHTERS SYNDROME". International Journal of Oncology 1.4 (1992): 459-465.
Chicago
MAHBOUDI, F., PHILLIPS, J., RAVECHE, E."IMMUNOGLOBULIN GENE SEQUENCE-ANALYSIS OF B-1 (CD5+B) CELL CLONES IN A MURINE MODEL OF CHRONIC LYMPHOCYTIC-LEUKEMIA AND RICHTERS SYNDROME". International Journal of Oncology 1, no. 4 (1992): 459-465. https://doi.org/10.3892/ijo.1.4.459