INHIBITION OF VIRAL MOS GENE-EXPRESSION IN TRANSFORMED-CELLS RESTRICTS CELL-CYCLE PROGRESSION THROUGH G1-PHASE AND G2-PHASE

  • Authors:
    • R HAMELIN
    • P PLANCHON
    • B SINGH
    • RB ARLINGHAUS
  • View Affiliations

  • Published online on: October 1, 1992     https://doi.org/10.3892/ijo.1.5.513
  • Pages: 513-523
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Abstract

The c-mos protein is required for the activation of M-phase promoting factor (MPF) during oocyte maturation and for MPF stabilization in unfertilized eggs. To address the question whether v-Mos plays an important role during cell cycle progression in transformed somatic cells, we have utilized a temperature-sensitive (ts) mutant of v-Mos. We examined NRK-6m2 cells chronically transformed by the ts mutant of Moloney murine sarcoma virus (MuSV ts110). NRK-6m2 cells transformed by MuSV ts 110 fail to express the viral mRNA for the heat-labile P85gag-mos protein at the restrictive temperature (39-degrees-C). To serve as controls for this study, two new transformation revertant cell lines, 6m3 and 6m4, were generated. 6m3 and 6m4 cells were found to express P85gag-mos maintained at both permissive (28-33-degrees-C) and restrictive temperatures. The growth rate of 6m2 cells at 37-degrees-C was significantly slower than either 6m3 or 6m4 cells at these same temperatures. To determine whether 6m2 cells maintained at 39-degrees-C were arrested at a specific phase in the cell cycle, flow cytometry analysis using double labeling was performed to quantitate cells in G0/G1, S- and G2/M-phases. The analyses indicated that 6m2 cells shifted to 39-degrees-C are arrested predominantly in the G1-phase of the cell-cycle. Of importance however, blocking v-mos expression also delayed progression through the G2-phase of the cell cycle.

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October 1992
Volume 1 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
HAMELIN R, PLANCHON P, SINGH B and ARLINGHAUS R: INHIBITION OF VIRAL MOS GENE-EXPRESSION IN TRANSFORMED-CELLS RESTRICTS CELL-CYCLE PROGRESSION THROUGH G1-PHASE AND G2-PHASE. Int J Oncol 1: 513-523, 1992.
APA
HAMELIN, R., PLANCHON, P., SINGH, B., & ARLINGHAUS, R. (1992). INHIBITION OF VIRAL MOS GENE-EXPRESSION IN TRANSFORMED-CELLS RESTRICTS CELL-CYCLE PROGRESSION THROUGH G1-PHASE AND G2-PHASE. International Journal of Oncology, 1, 513-523. https://doi.org/10.3892/ijo.1.5.513
MLA
HAMELIN, R., PLANCHON, P., SINGH, B., ARLINGHAUS, R."INHIBITION OF VIRAL MOS GENE-EXPRESSION IN TRANSFORMED-CELLS RESTRICTS CELL-CYCLE PROGRESSION THROUGH G1-PHASE AND G2-PHASE". International Journal of Oncology 1.5 (1992): 513-523.
Chicago
HAMELIN, R., PLANCHON, P., SINGH, B., ARLINGHAUS, R."INHIBITION OF VIRAL MOS GENE-EXPRESSION IN TRANSFORMED-CELLS RESTRICTS CELL-CYCLE PROGRESSION THROUGH G1-PHASE AND G2-PHASE". International Journal of Oncology 1, no. 5 (1992): 513-523. https://doi.org/10.3892/ijo.1.5.513