Growth suppression of non-small cell lung carcinoma cells by the introduction of the p16(INK4A) gene

  • Authors:
    • J Adachi
    • T Kohno
    • G Baeg
    • T Akiyama
    • J Yokota
  • View Affiliations

  • Published online on: January 1, 1997     https://doi.org/10.3892/ijo.10.1.33
  • Pages: 33-39
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The p16(INK4A) gene is frequently inactivated in nonsmall cell lung carcinoma (NSCLC) by either mutations, deletions or DNA methylations. To assess the biological significance of p16(INK4A) inactivation in the development of NSCLC, full-length p16(INK4A) cDNA was introduced into NSCLC cell lines, A549 and H322, in which p16(INK4A) was homozygously deleted. NSCLC cells transfected with the p16(INK4A) expression vector formed colonies in 20-68% of those with a control vector, and exogenous p16(INK4A) protein was expressed in 4 of 68 A549-derived clones and none of 29 H322-derived clones, respectively. A549-derived clones which stably expressed the exogenous p16(INK4A) gene showed significant decrease in growth rate in vitro and tumorigenicity in vivo in proportion to the level of p16(INK4A) expression. Furthermore, the cell cycle of these cells significantly delayed with accumulation of cells in G1 phase. Micro-injection of p16(INK4A) expression vector also revealed that p16(INK4A) blocked S phase entry in both A549 and H322 cells. These results suggest that the restoration of the p16(INK4A) function suppresses the growth of NSCLC cells by induction of G1 arrest in the cells. Therefore, inactivation of p16(INK4A) may play an important role in the enhancement of unregulated NSCLC growth in vivo.

Related Articles

Journal Cover

January 1997
Volume 10 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Adachi J, Kohno T, Baeg G, Akiyama T and Yokota J: Growth suppression of non-small cell lung carcinoma cells by the introduction of the p16(INK4A) gene. Int J Oncol 10: 33-39, 1997
APA
Adachi, J., Kohno, T., Baeg, G., Akiyama, T., & Yokota, J. (1997). Growth suppression of non-small cell lung carcinoma cells by the introduction of the p16(INK4A) gene. International Journal of Oncology, 10, 33-39. https://doi.org/10.3892/ijo.10.1.33
MLA
Adachi, J., Kohno, T., Baeg, G., Akiyama, T., Yokota, J."Growth suppression of non-small cell lung carcinoma cells by the introduction of the p16(INK4A) gene". International Journal of Oncology 10.1 (1997): 33-39.
Chicago
Adachi, J., Kohno, T., Baeg, G., Akiyama, T., Yokota, J."Growth suppression of non-small cell lung carcinoma cells by the introduction of the p16(INK4A) gene". International Journal of Oncology 10, no. 1 (1997): 33-39. https://doi.org/10.3892/ijo.10.1.33