Establishment and characterization of ovarian endometrioid adenocarcinoma cell line in nude mice and analyses of the immunohistochemical property among the original, recurred, and heterotransplanted tumor
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- Published online on: February 1, 1997 https://doi.org/10.3892/ijo.10.2.311
- Pages: 311-316
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Abstract
The establishment and characterization of a new human endometrioid adenocarcinoma cell line (KOC-8e) derived from the malignant ascites is described. The original tumor at the initial operation showed positive in TPA, and SLX immunohistochemically. By contrast, recurred tumor showed positive for CA125, TPA, SLX, MUC-1, and p53. In addition, the heterotransplanted tumor showed positive for CA125, TPA, SLX, MUG-1, and p53. The DNA index was similar in the primary, recurred, and nude mouse tumor ranging between 1.93 and 2.05. The tumor growths were suppressed dose dependently by CDDP and CPT-11. CA125 showed useful as a tumor marker of this tumor, however, the nude mice had detectable tumor without elevation of CA125 after low dose application of CDDP and CPT-11. Thus, clinical determination of chemotherapeutic effect and residual tumor cannot be made only by CA125. KOC-8e cells could be useful to study histological analysis and chemosensitivity.
