Inhibition of the GTP-dependent polymerization of tubulin by methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME)
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- Published online on: May 1, 1997 https://doi.org/10.3892/ijo.10.5.911
- Pages: 911-913
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Abstract
The hormonally inactive thyroid hormone analog methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME) at 1-5 mu M concentrations inhibits the GTP-dependent polymerization of MTP as determined by an optical test. This inhibition is critically dependent on the concentration of GTP. The quantitative correlation between the concentrations of DIME and GTP, under conditions of a linear rat of MTP polymerization, follows Michaelis-Menten kinetics and the inhibition portrays a 'mixed' type, where k(m) for GTP and V-max are altered simultaneously. Chemical analogs of DIME inhibit MTP polymerization parallel to their antitumorigenic action in vivo. The MTP site is one of the early cellular response sites of DIME.
