Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells

  • Authors:
    • F Van den Brûle
    • A Bellahcene
    • P Jackers
    • F Liu
    • M Sobel
    • V Castronovo
  • View Affiliations

  • Published online on: August 1, 1997     https://doi.org/10.3892/ijo.11.2.261
  • Pages: 261-264
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Abstract

Galectin-3 is a 30-kDa galactose-binding protein member of the galectin family. Galectin-3 is involved in multiple intracellular and extracellular biological functions, e.g. interactions with laminin and with nucleic acids. This latter property is consistent with the presence of 3. serum-response factor-like domain at the amino-terminal part of the protein. Galectin-3 expression is upregulated during serum-mediated induction of proliferation. In order to examine the role of galectin-3 in breast cancer cell proliferation, we examined in this study the influence of antisense galectin-3 complementary DNA stable transfection on the in vitro thymidine incorporation of human breast cancer MDA-MB435 cells. Two stable transfectants, clones 5.24 and 5.29, were selected based both on the presence of a complete CMV promoter-antisense galectin-3 cDNA cassette as assayed by polymerase chain reaction, and on efficient down-regulation of galectin-3 protein expression as determined by Western blotting. Thymidine incorporation experiments showed that both clones were characterized by significantly decreased values of DNA incorporation compared to wild-type transfectants (55 to 68%, and 71 to 82% of the control clone values). Our data demonstrate for the first time that galectin-3 decreases thymidine incorporation in breast cancer cells. The mechanism underlying this property of galectin-3 and its importance during breast cancer development remain to be elucidated.

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August 1997
Volume 11 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Van den Brûle F, Bellahcene A, Jackers P, Liu F, Sobel M and Castronovo V: Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. Int J Oncol 11: 261-264, 1997
APA
Van den Brûle, F., Bellahcene, A., Jackers, P., Liu, F., Sobel, M., & Castronovo, V. (1997). Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. International Journal of Oncology, 11, 261-264. https://doi.org/10.3892/ijo.11.2.261
MLA
Van den Brûle, F., Bellahcene, A., Jackers, P., Liu, F., Sobel, M., Castronovo, V."Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells". International Journal of Oncology 11.2 (1997): 261-264.
Chicago
Van den Brûle, F., Bellahcene, A., Jackers, P., Liu, F., Sobel, M., Castronovo, V."Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells". International Journal of Oncology 11, no. 2 (1997): 261-264. https://doi.org/10.3892/ijo.11.2.261