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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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October 1997 Volume 11 Issue 4

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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October 1997 Volume 11 Issue 4

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Article

Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells

  • Authors:
    • M Sharif
    • T Sharif
    • M Dilling
    • H Hosoi
    • J Lawrence
    • P Houghton

  • View Affiliations / Copyright

    Affiliations: UNIV VIRGINIA,SCH MED,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908.
  • Pages: 797-805
    |
    Published online on: October 1, 1997
       https://doi.org/10.3892/ijo.11.4.797
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Abstract

The mitogenic substance P receptor (NK-1 subtype) is expressed in many primary human tumors with the highest frequency of expression appearing in astrocytomas and glioblastomas (75% and 100%, respectively). Recently, we showed that substance P neuropeptide induces DNA synthesis in the human astrocytoma U-373MG cells by activating the mitogen-activated protein (MAP) kinase pathway leading to the induction of c-Fos and c-Myc expression. The induction of these immediate early genes is necessary for the progression of cells form G1 to S phase of the cell cycle. In this study, we demonstrate that U-373MG cells are highly sensitive to the growth-inhibitory action of rapamycin at nanomolar concentrations (IC50 <1 ng/ml). We also show that SP peptide stimulates protein synthesis in the U-373MG cell line by activating a rapamycin-sensitive signaling pathway. Further, we demonstrate that SP is potent in stimulating PHAS-I protein (also known as 4E-BP1) phosphorylation and p70 S6 kinase (p70(S6K)) phosphorylation and enzymatic activity, and that this stimulation is inhibited by subnanomolar concentrations of rapamycin. In contrast, rapamycin was not at all effective in repressing SP-induced activation of MAP kinase pathway, c-Fos phosphoprotein expression, and DNA synthesis in U-373MG astrocytoma cells.

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Copy and paste a formatted citation
Spandidos Publications style
Sharif M, Sharif T, Dilling M, Hosoi H, Lawrence J and Houghton P: Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells. Int J Oncol 11: 797-805, 1997.
APA
Sharif, M., Sharif, T., Dilling, M., Hosoi, H., Lawrence, J., & Houghton, P. (1997). Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells. International Journal of Oncology, 11, 797-805. https://doi.org/10.3892/ijo.11.4.797
MLA
Sharif, M., Sharif, T., Dilling, M., Hosoi, H., Lawrence, J., Houghton, P."Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells". International Journal of Oncology 11.4 (1997): 797-805.
Chicago
Sharif, M., Sharif, T., Dilling, M., Hosoi, H., Lawrence, J., Houghton, P."Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells". International Journal of Oncology 11, no. 4 (1997): 797-805. https://doi.org/10.3892/ijo.11.4.797
Copy and paste a formatted citation
x
Spandidos Publications style
Sharif M, Sharif T, Dilling M, Hosoi H, Lawrence J and Houghton P: Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells. Int J Oncol 11: 797-805, 1997.
APA
Sharif, M., Sharif, T., Dilling, M., Hosoi, H., Lawrence, J., & Houghton, P. (1997). Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells. International Journal of Oncology, 11, 797-805. https://doi.org/10.3892/ijo.11.4.797
MLA
Sharif, M., Sharif, T., Dilling, M., Hosoi, H., Lawrence, J., Houghton, P."Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells". International Journal of Oncology 11.4 (1997): 797-805.
Chicago
Sharif, M., Sharif, T., Dilling, M., Hosoi, H., Lawrence, J., Houghton, P."Rapamycin inhibits substance P-induced protein synthesis and phosphorylation of PHAS-I (4E-BP1) and p70 S6 kinase (p70(S6K)) in human astrocytoma cells". International Journal of Oncology 11, no. 4 (1997): 797-805. https://doi.org/10.3892/ijo.11.4.797
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