Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines

  • Authors:
    • M Loprevite
    • L Varesco
    • R Favoni
    • G Ferrara
    • F Moro
    • L Ottaggio
    • G Fronza
    • P Campomenosi
    • A Abbondandolo
    • G Cutrona
    • S Roncella
    • A Albini
    • M Aluigi
    • S Pozzi
    • C Pera
    • R Biticchi
    • V Gismondi
    • F Grossi
    • M Pennucci
    • A Ardizzoni
  • View Affiliations

  • Published online on: December 1, 1997     https://doi.org/10.3892/ijo.11.6.1203
  • Pages: 1203-1208
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Abstract

Six non-small cell lung cancer (NSCLC) cell lines (A-549, Ca-Lu-6, SK-Lu-1, Ca-Lu-1, SK-Mes-1 and LX-1) were studied to assess the presence of multiple concomitant alterations of different oncogenes (K-ras, bcl-2) and tumor suppressor genes (p53, Rb) in NSCLC. K-ras (exon 1) and p53 (exons 5-8) gene mutations were determined via a PCR-based-DGGE (Denaturing Gradient Gel Electro-phoresis) and by sequencing approach. Different mutations were found in the Ist exon of K-ras gene in 5 of 6 cell lines examined. Five of six cell lines contained K-ras mutations at codon 12 (A-549, SK-Lu-1, LX-1) or codon 13 (SK-Mes-1, Ca-Lu-1). In addition, 5 of 6 cell lines showed p53 mutations of exon 8 (SK-Mes-1, Ca-Lu-1 cod. 280; LX-1 cod. 273) or exon 6 (Ca-Lu-6 cod. 196; SK-Lu-1 cod. 193). In 4 of these cell lines, p53 protein nuclear expression was also confirmed with DO-7 mAb immunocytochemistry. Expression of cytoplasmic bcl-2 protein, by anti-bcl-2 mAb flow cytometric analysis, was found in A-549, Ca-Lu-1, SK-Lu-1, SK-Mes-1 cell lines. In contrast, RT-PCR analysis of Rb gene could not identify any change in the cell lines examined. In conclusion, most NSCLC cell lines tested displayed concomitant multiple oncogene/tumor suppressor gene alterations.

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December 1997
Volume 11 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Loprevite M, Varesco L, Favoni R, Ferrara G, Moro F, Ottaggio L, Fronza G, Campomenosi P, Abbondandolo A, Cutrona G, Cutrona G, et al: Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines. Int J Oncol 11: 1203-1208, 1997
APA
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L. ... Ardizzoni, A. (1997). Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines. International Journal of Oncology, 11, 1203-1208. https://doi.org/10.3892/ijo.11.6.1203
MLA
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L., Fronza, G., Campomenosi, P., Abbondandolo, A., Cutrona, G., Roncella, S., Albini, A., Aluigi, M., Pozzi, S., Pera, C., Biticchi, R., Gismondi, V., Grossi, F., Pennucci, M., Ardizzoni, A."Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines". International Journal of Oncology 11.6 (1997): 1203-1208.
Chicago
Loprevite, M., Varesco, L., Favoni, R., Ferrara, G., Moro, F., Ottaggio, L., Fronza, G., Campomenosi, P., Abbondandolo, A., Cutrona, G., Roncella, S., Albini, A., Aluigi, M., Pozzi, S., Pera, C., Biticchi, R., Gismondi, V., Grossi, F., Pennucci, M., Ardizzoni, A."Analysis of K-ras, p53, bcl-2 and Rb expression in non-small cell lung cancer cell lines". International Journal of Oncology 11, no. 6 (1997): 1203-1208. https://doi.org/10.3892/ijo.11.6.1203