Tumour vaccines expressing IL-2, CD80, and IL-2 plus CD80 gene

  • Authors:
    • J Bubenik
    • P Rossner
    • D Bubenikova
    • J Simova
    • M Indrova
    • E Sloncova
  • View Affiliations

  • Published online on: December 1, 1997     https://doi.org/10.3892/ijo.11.6.1213
  • Pages: 1213-1219
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Abstract

Experiments were designed to investigate immunogenicity and therapeutic efficacy of tumour vaccines constructed by transfection of poorly immunogenic murine sarcoma Mc12 with synergistic CD80 and IL-2 genes. Immunization/challenge experiments demonstrated that both, IL-2(+) and IL-2(+) plus CD80(+) live cell vaccines can exert an immunizing stimulus, the IL-2(+) plus CD80(+) vaccine being superior to the IL-2(+) vaccine. Live CD80(+) Mc12 cell vaccine could not be tested, since the vaccine was highly tumorigenic in the doses required for immunization. Preimmunization with IL-2(+) and IL-2(+) plus CD80(+) vaccines induced regressions of a proportion of the parental Mc12 challenge inocula after their temporary growth. Areas of necrosis and extensive infiltration with Mac1(+) and CD4(+) leukocytes have been observed in the regressing sarcomas. When the therapeutic efficacy of the irradiated CD80(+), IL-2(+), and mixed CD80(+) plus IL-2(+) vaccines was compared, it was found that the insertion of the IL-2, but not CD80 gene alone was efficient. The mixed IL-2(+) plus CD80(+) tumour vaccine was able to protect and prolong survival of a higher proportion of mice than the IL-2(+) tumour vaccine.

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December 1997
Volume 11 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Bubenik J, Rossner P, Bubenikova D, Simova J, Indrova M and Sloncova E: Tumour vaccines expressing IL-2, CD80, and IL-2 plus CD80 gene. Int J Oncol 11: 1213-1219, 1997
APA
Bubenik, J., Rossner, P., Bubenikova, D., Simova, J., Indrova, M., & Sloncova, E. (1997). Tumour vaccines expressing IL-2, CD80, and IL-2 plus CD80 gene. International Journal of Oncology, 11, 1213-1219. https://doi.org/10.3892/ijo.11.6.1213
MLA
Bubenik, J., Rossner, P., Bubenikova, D., Simova, J., Indrova, M., Sloncova, E."Tumour vaccines expressing IL-2, CD80, and IL-2 plus CD80 gene". International Journal of Oncology 11.6 (1997): 1213-1219.
Chicago
Bubenik, J., Rossner, P., Bubenikova, D., Simova, J., Indrova, M., Sloncova, E."Tumour vaccines expressing IL-2, CD80, and IL-2 plus CD80 gene". International Journal of Oncology 11, no. 6 (1997): 1213-1219. https://doi.org/10.3892/ijo.11.6.1213