P-glycoprotein-mediated multidrug resistance is modulated by pretreatment with chemosensitizers in HCT-8 carcinoma cells in vitro.

  • Authors:
    • I Haberl
    • H Swatonek
    • K Schaufler
    • E Ulsperger
    • E Wenzl
    • G Theyer
    • G Hamilton
    • T Thalhammer
  • View Affiliations

  • Published online on: May 1, 1998     https://doi.org/10.3892/ijo.12.5.1137
  • Pages: 1137-1179
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Abstract

The effects of pretreatment with the multidrug resistance (MDR) modulators verapamil (VPM), tamoxifen (TMX), cyclosporin A (CsA), and SDZ PSC833 (PSC) on drug sensitivity of the P-glycoprotein (Pgp) expressing human ileocecal carcinoma cell line HCT-8 is described. Following pretreatment of 2, 16 and 48 h with the individual modulators, rhodamine 123 efflux (RHO), transepithelial vinblastine transport (VIN) across treated HCT-8 monolayers, and chemosensitivity to doxorubicin (DOX) were determined and compared to Pgp protein expression and phosphorylation. After 2 h, VPM, TMX, CsA and PSC inhibited RHO efflux and VIN transport and increased the chemosensitivity of HCT-8 to DOX significantly. Prolonged exposure failed to further increase inhibition of Pgp-mediated transport, but in contrast maximized phosphorylation of Pgp (16 h) and Pgp protein expression (48 h), respectively.

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May 1998
Volume 12 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Haberl I, Swatonek H, Schaufler K, Ulsperger E, Wenzl E, Theyer G, Hamilton G and Thalhammer T: P-glycoprotein-mediated multidrug resistance is modulated by pretreatment with chemosensitizers in HCT-8 carcinoma cells in vitro.. Int J Oncol 12: 1137-1179, 1998.
APA
Haberl, I., Swatonek, H., Schaufler, K., Ulsperger, E., Wenzl, E., Theyer, G. ... Thalhammer, T. (1998). P-glycoprotein-mediated multidrug resistance is modulated by pretreatment with chemosensitizers in HCT-8 carcinoma cells in vitro.. International Journal of Oncology, 12, 1137-1179. https://doi.org/10.3892/ijo.12.5.1137
MLA
Haberl, I., Swatonek, H., Schaufler, K., Ulsperger, E., Wenzl, E., Theyer, G., Hamilton, G., Thalhammer, T."P-glycoprotein-mediated multidrug resistance is modulated by pretreatment with chemosensitizers in HCT-8 carcinoma cells in vitro.". International Journal of Oncology 12.5 (1998): 1137-1179.
Chicago
Haberl, I., Swatonek, H., Schaufler, K., Ulsperger, E., Wenzl, E., Theyer, G., Hamilton, G., Thalhammer, T."P-glycoprotein-mediated multidrug resistance is modulated by pretreatment with chemosensitizers in HCT-8 carcinoma cells in vitro.". International Journal of Oncology 12, no. 5 (1998): 1137-1179. https://doi.org/10.3892/ijo.12.5.1137