Exogenous thrombospondin stimulates the proliferation of non-thrombospondin-producing cells.

  • Authors:
    • Y Yamashita
    • S Sendo
    • T Hosokawa
    • G Tuszynski
    • T Kurohiji
    • R Beppu
    • T Shinohara
    • T Kinugasa
    • T Shirakusa
  • View Affiliations

  • Published online on: August 1, 1998     https://doi.org/10.3892/ijo.13.2.355
  • Pages: 355-364
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Abstract

The effects of thrombospondin (TSP) on the proliferation of two different human carcinoma cell lines (KIM-1 and CW-2) were investigated. The characterization of these two carcinoma cells by immunohistochemistry using anti-TSP antibody and anti-TSP-receptor antibody showed that the KIM-1 had TSP-receptors and TSP, while the CW-2 had only TSP-receptors. The addition of exogenous TSP (10 or 20 microg/ml) to culture medium stimulated the cell proliferation of CW-2 but not that of KIM-1. The cell count for CW-2 was increased dosage-dependently from 10.3 0.6x104/ml at zero TSP concentration to 12.9 0.6x104/ml at 10 microg/ml TSP concentration and to 14.7 0. 4x104/ml at 20 microg/ml TSP (each p<0.0001). In conclusion, though TSP promoted the proliferation of non-TSP-producing cells, it did not promote proliferation of TSP-producing cells. Therefore, it is predicted that TSP was already at saturated activity concentration in the TSP-producing cell line (KIM-1).

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Aug 1998
Volume 13 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yamashita Y, Sendo S, Hosokawa T, Tuszynski G, Kurohiji T, Beppu R, Shinohara T, Kinugasa T and Shirakusa T: Exogenous thrombospondin stimulates the proliferation of non-thrombospondin-producing cells.. Int J Oncol 13: 355-364, 1998
APA
Yamashita, Y., Sendo, S., Hosokawa, T., Tuszynski, G., Kurohiji, T., Beppu, R. ... Shirakusa, T. (1998). Exogenous thrombospondin stimulates the proliferation of non-thrombospondin-producing cells.. International Journal of Oncology, 13, 355-364. https://doi.org/10.3892/ijo.13.2.355
MLA
Yamashita, Y., Sendo, S., Hosokawa, T., Tuszynski, G., Kurohiji, T., Beppu, R., Shinohara, T., Kinugasa, T., Shirakusa, T."Exogenous thrombospondin stimulates the proliferation of non-thrombospondin-producing cells.". International Journal of Oncology 13.2 (1998): 355-364.
Chicago
Yamashita, Y., Sendo, S., Hosokawa, T., Tuszynski, G., Kurohiji, T., Beppu, R., Shinohara, T., Kinugasa, T., Shirakusa, T."Exogenous thrombospondin stimulates the proliferation of non-thrombospondin-producing cells.". International Journal of Oncology 13, no. 2 (1998): 355-364. https://doi.org/10.3892/ijo.13.2.355