Differential transferrin receptor density in human colorectal cancer: A potential probe for diagnosis and therapy.

Prost,A C; Ménégaux,F; Langlois,P; Vidal,J M; Koulibaly,M; Jost,J L; Duron,J J; Chigot,J P; Vayre,P; Aurengo,A; Legrand,J C; Rosselin,G; Gespach,C

doi:10.3892/ijo.13.4.871

Differential transferrin receptor density in human colorectal cancer: A potential probe for diagnosis and therapy.

Authors:
- A C Prost
- F Ménégaux
- P Langlois
- J M Vidal
- M Koulibaly
- J L Jost
- J J Duron
- J P Chigot
- P Vayre
- A Aurengo
- J C Legrand
- G Rosselin
- C Gespach
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Affiliations: Service Central de Medecine Nucleaire, Centre Hospitalier Le Mans, 72037 Le-Mans, France.
Published online on: October 1, 1998 https://doi.org/10.3892/ijo.13.4.871
Pages: 871-876

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Abstract

Transferrin receptor density was investigated in human colorectal surgical specimens. Crude membranes were prepared from 23 cancer tumors (adenocarcinoma or malignant villous tumor) and 3 non-cancer tumors (polyadenoma or villous tumor) and 26 adjacent control mucosa. Contrary to non-cancer tumors, Scatchard analysis of 125I-transferrin binding data evidenced higher maximal transferrin binding capacity and lower dissociation constant in cancer tissues (Bmax cancer 1.828+/-0.320 nmol/g, Kd 24.1+/-4.7 nM), as compared to paired control colonic mucosa (Bmax contol 0.851+/-0.182 nmol/g, Kd 30.7+/-7.3 nM), paired t-tests: Bmax p<0.001, Kd p<0.05). As the cancer/control Bmax ratio was 2.6+/-0.4,transferrin carrier constructs should be proposed for cancer imaging or therapy.