NRG-3 in human breast cancers: activation of multiple erbB family proteins.

  • Authors:
    • M M Hijazi
    • P E Young
    • M K Dougherty
    • D S Bressette
    • T T Cao
    • J H Pierce
    • L M Wong
    • M Alimandi
    • C R King
  • View Affiliations

  • Published online on: November 1, 1998     https://doi.org/10.3892/ijo.13.5.1061
  • Pages: 1061-1068
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Abstract

Ligands of the EGF/Heregulin family control the growth of epithelial cells by binding to receptors of the erbB family. By searching a large database of cDNA sequences at Human Genome Sciences Inc. we have identified a new encoded protein sequence containing all the conserved elements of the EGF/Heregulin family. The same sequence has recently been independently identified as NRG-3. The EGF-like domain of NRG-3 was generated as a recombinant protein in E. coli and used to test the specificity of receptor binding. In human breast cancer cells and in 32D cells transfected by erbB family members, NRG-3 activated multiple erbB family members. These include EGF receptor (erbB1) and erbB4 when expressed individually and erbB2 and erbB3 when expressed together. Recombinant NRG-3 altered the growth of human breast cancer cells growing in vitro. NRG-3 was expressed in cell lines derived from breast cancer. These results indicate that NRG-3 is a potential regulator of normal and malignant breast epithelial cells in vivo.

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Nov 1998
Volume 13 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hijazi M, Young P, Dougherty M, Bressette D, Cao T, Pierce J, Wong L, Alimandi M and King C: NRG-3 in human breast cancers: activation of multiple erbB family proteins.. Int J Oncol 13: 1061-1068, 1998.
APA
Hijazi, M., Young, P., Dougherty, M., Bressette, D., Cao, T., Pierce, J. ... King, C. (1998). NRG-3 in human breast cancers: activation of multiple erbB family proteins.. International Journal of Oncology, 13, 1061-1068. https://doi.org/10.3892/ijo.13.5.1061
MLA
Hijazi, M., Young, P., Dougherty, M., Bressette, D., Cao, T., Pierce, J., Wong, L., Alimandi, M., King, C."NRG-3 in human breast cancers: activation of multiple erbB family proteins.". International Journal of Oncology 13.5 (1998): 1061-1068.
Chicago
Hijazi, M., Young, P., Dougherty, M., Bressette, D., Cao, T., Pierce, J., Wong, L., Alimandi, M., King, C."NRG-3 in human breast cancers: activation of multiple erbB family proteins.". International Journal of Oncology 13, no. 5 (1998): 1061-1068. https://doi.org/10.3892/ijo.13.5.1061