Enhancement of all-trans-retinoic acid efficiency in granulocytic differentiation of HL-60 cells by incorporation into low density lipoprotein.

Gueddari,N; Bobichon,H; Depierreux,C; Liautaud-Roger,F; Duriez,P; Dufer,J; Jardillier,J C

doi:10.3892/ijo.13.5.1069

Enhancement of all-trans-retinoic acid efficiency in granulocytic differentiation of HL-60 cells by incorporation into low density lipoprotein.

Authors:
- N Gueddari
- H Bobichon
- C Depierreux
- F Liautaud-Roger
- P Duriez
- J Dufer
- J C Jardillier
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Affiliations: Laboratoire de Biochimie, IFR53 Biomolecules, EA 2063, UFR Pharmacie, 51100 Reims, France.
Published online on: November 1, 1998 https://doi.org/10.3892/ijo.13.5.1069
Pages: 1069-1144

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Abstract

All-trans-retinoic acid (ATRA) has been proven to lead to complete remission of acute promyelocytic leukemia by inducing differentiation into granulocytes except when an acquired resistance occurred. High levels of low density lipoprotein (LDL) receptor in cancer cells suggested the use of ATRA incorporated into LDL. 50% of HL-60 cell differentiation were obtained with 5 nmoles/l of ATRA-LDL compared to 150 moles/l of ATRA. Maximal differentiation (80%) was reached at 25 nmoles/l and 1,000 nmoles/l respectively. This higher efficiency suggests the involvement of LDL receptor in ATRA-LDL internalization and/or the protection of the drug, from eventual catabolism, by LDL particles.