Cisplatin and phorbol ester independently induce ERCC-1 protein in human ovarian carcinoma cells.

  • Authors:
    • Q Li
    • L Ding
    • J J Yu
    • C Mu
    • B Tsang
    • F Bostick-Bruton
    • E Reed
  • View Affiliations

  • Published online on: November 1, 1998     https://doi.org/10.3892/ijo.13.5.987
  • Pages: 987-1079
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Abstract

Nucleotide excision repair (NER) is the DNA repair pathway by which cisplatin-induced damage is removed from DNA in human cells. ERCC-1 is one of the essential proteins in NER, and is essential for life. Enhanced ERCC-1 expression has been associated with clinical and cellular resistance to cisplatin. We therefore carried out this study to investigate the effect of cisplatin on ERCC-1 protein expression in A2780/CP70 human ovarian cancer cells. Western blot analysis showed that ERCC-1 protein levels were increased to more than 3 times control after a 1 h cisplatin exposure to A2780/CP70 cells in culture. This increase was time- and concentration-dependent. The effect of cisplatin was maximal at 40 mM and peaked 24-48 h after exposure to the drug. These results extend our previous observations that ERCC-1 mRNA expression is induced by cisplatin in this system. TPA, a known AP-1 activator and tumor-promoting phorbol ester, also induced ERCC-1 protein to the same extent as cisplatin, but did not synergize with cisplatin in this regard. These findings suggest that ERCC-1 gene up-regulation in these cells can result through a DNA damage-response pathway, or through the induction of AP-1 activity, independent of the occurrence of DNA damage.

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Nov 1998
Volume 13 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li Q, Ding L, Yu J, Mu C, Tsang B, Bostick-Bruton F and Reed E: Cisplatin and phorbol ester independently induce ERCC-1 protein in human ovarian carcinoma cells.. Int J Oncol 13: 987-1079, 1998.
APA
Li, Q., Ding, L., Yu, J., Mu, C., Tsang, B., Bostick-Bruton, F., & Reed, E. (1998). Cisplatin and phorbol ester independently induce ERCC-1 protein in human ovarian carcinoma cells.. International Journal of Oncology, 13, 987-1079. https://doi.org/10.3892/ijo.13.5.987
MLA
Li, Q., Ding, L., Yu, J., Mu, C., Tsang, B., Bostick-Bruton, F., Reed, E."Cisplatin and phorbol ester independently induce ERCC-1 protein in human ovarian carcinoma cells.". International Journal of Oncology 13.5 (1998): 987-1079.
Chicago
Li, Q., Ding, L., Yu, J., Mu, C., Tsang, B., Bostick-Bruton, F., Reed, E."Cisplatin and phorbol ester independently induce ERCC-1 protein in human ovarian carcinoma cells.". International Journal of Oncology 13, no. 5 (1998): 987-1079. https://doi.org/10.3892/ijo.13.5.987