Interferon regulatory factor-1 gene abnormality and loss of growth inhibitory effect of interferon-alpha in human hepatoma cell lines.

  • Authors:
    • S Tada
    • H Saito
    • S Tsunematsu
    • H Ebinuma
    • K Wakabayashi
    • T Masuda
    • H Ishii
  • View Affiliations

  • Published online on: December 1, 1998     https://doi.org/10.3892/ijo.13.6.1207
  • Pages: 1207-1223
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Abstract

The effect of IFN-alpha on the growth of 5 hepatoma cell lines and a normal liver-derived cell line were examined. IFN dose-dependently inhibited the growth of cell lines except for HLE and PLC/PRF/5 in which the inhibition only occurred at a high concentration over 10,000 IU/ml. IFN-alpha induced the G1 arrest of these cells according to upregulation of p21WAF-1 expression, which is induced in PLC/PRF/5 and HLE only at a high IFN concentration. The receptor for IFN-alpha was well expressed in all the cell lines. DNA rearrangement of IRF-1 was detected in HLE and PLC/PRF/5 by Southern blotting, while IRF-2 gene was preserved. IFN-induced gene expressions were compared between HCC-T, HCC-M and PLC/PRF/5. RT-PCR demonstrated that the full-length IRF-1 and -2 mRNA was transcribed in all cell lines, but the mRNA amount of former gene in PLC/PRF/5 was less than that in HCC-T and HCC-M, about 1/10 by competitive RT-PCR. The sequence analysis of IRF-1 cDNA was performed and the full-length mRNA transcription was reconfirmed in PLC/PRF/5, but no significant point mutation was detected. These results suggest that IFN-alpha inhibits hepatoma growth by increasing p21WAF-1 expression only when the IRF-1 gene is preserved normally.

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Dec 1998
Volume 13 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tada S, Saito H, Tsunematsu S, Ebinuma H, Wakabayashi K, Masuda T and Ishii H: Interferon regulatory factor-1 gene abnormality and loss of growth inhibitory effect of interferon-alpha in human hepatoma cell lines.. Int J Oncol 13: 1207-1223, 1998.
APA
Tada, S., Saito, H., Tsunematsu, S., Ebinuma, H., Wakabayashi, K., Masuda, T., & Ishii, H. (1998). Interferon regulatory factor-1 gene abnormality and loss of growth inhibitory effect of interferon-alpha in human hepatoma cell lines.. International Journal of Oncology, 13, 1207-1223. https://doi.org/10.3892/ijo.13.6.1207
MLA
Tada, S., Saito, H., Tsunematsu, S., Ebinuma, H., Wakabayashi, K., Masuda, T., Ishii, H."Interferon regulatory factor-1 gene abnormality and loss of growth inhibitory effect of interferon-alpha in human hepatoma cell lines.". International Journal of Oncology 13.6 (1998): 1207-1223.
Chicago
Tada, S., Saito, H., Tsunematsu, S., Ebinuma, H., Wakabayashi, K., Masuda, T., Ishii, H."Interferon regulatory factor-1 gene abnormality and loss of growth inhibitory effect of interferon-alpha in human hepatoma cell lines.". International Journal of Oncology 13, no. 6 (1998): 1207-1223. https://doi.org/10.3892/ijo.13.6.1207