Constitutive and IFN-gamma regulated expression of IL-7 and IL-15 in human renal cell cancer.

  • Authors:
    • P Trinder
    • U Seitzer
    • J Gerdes
    • B Seliger
    • M Maeurer
  • View Affiliations

  • Published online on: January 1, 1999     https://doi.org/10.3892/ijo.14.1.23
  • Pages: 23-54
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Abstract

Although not structurally related, the pleiotropic cytokines interleukin-7 (IL-7) and interleukin-15 (IL-15) share a variety of biological functions including stimulation and maintenance of cellular immune responses. Cytokines, such as IL-7 or IL-15, elaborated by cells in situ, e.g. cancer cells, may be involved in shaping the quality of anti-tumor directed immune responses. We have analysed the constitutive and IFN-gamma-inducible expression of IL-15 or IL-7 mRNA, protein expression, and protein secretion in human tumor cell lines of distinct origin. IL-15 mRNA expression was detected in renal cell carcinoma (RCC), small cell lung carcinoma (SCLC), glioblastoma, neuroblastoma, mesothelioma cells and in EBV-transformed B-lymphocytes. IL-7-specific transcripts could be detected in colorectal cancer and in renal cell cancer cell lines. Immunohistochemical analysis demonstrated cytosolic IL-15 protein expression in renal cell cancer cells without apparent IL-15 protein secretion in vitro. Time kinetic analyses revealed that IFN-gamma mediated increase of IL-15 mRNA expression was transcriptionally regulated and dependent on de novo protein synthesis. However, enhanced IL-15 mRNA expression did not lead to effective protein secretion. In contrast, IL-7 mRNA expression in renal cell cancer or in colorectal cancer was associated with effective protein secretion which could be augmented by IFNgamma-treatment. These data suggest that both IL-7 and IL-15 mRNA are expressed in renal cell cancer, but exclusively IL-7 may be elaborated by tumor cells in situ. IL-15 regulation appears to be tightly controlled both at the transciptional and post-transcriptional level. Appropriate stimuli leading to effective IL-15 secretion from tumor cells may aid in modulating cellular immune responses directed against cancer.

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Jan 1999
Volume 14 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Trinder P, Seitzer U, Gerdes J, Seliger B and Maeurer M: Constitutive and IFN-gamma regulated expression of IL-7 and IL-15 in human renal cell cancer.. Int J Oncol 14: 23-54, 1999
APA
Trinder, P., Seitzer, U., Gerdes, J., Seliger, B., & Maeurer, M. (1999). Constitutive and IFN-gamma regulated expression of IL-7 and IL-15 in human renal cell cancer.. International Journal of Oncology, 14, 23-54. https://doi.org/10.3892/ijo.14.1.23
MLA
Trinder, P., Seitzer, U., Gerdes, J., Seliger, B., Maeurer, M."Constitutive and IFN-gamma regulated expression of IL-7 and IL-15 in human renal cell cancer.". International Journal of Oncology 14.1 (1999): 23-54.
Chicago
Trinder, P., Seitzer, U., Gerdes, J., Seliger, B., Maeurer, M."Constitutive and IFN-gamma regulated expression of IL-7 and IL-15 in human renal cell cancer.". International Journal of Oncology 14, no. 1 (1999): 23-54. https://doi.org/10.3892/ijo.14.1.23