Low incidence of p53 mutations in betel quid and tobacco chewing-associated oral squamous carcinoma from India.

  • Authors:
    • K Kannan
    • A K Munirajan
    • J Krishnamurthy
    • V Bhuvarahamurthy
    • B K Mohanprasad
    • K H Panishankar
    • N Tsuchida
    • G Shanmugam
  • View Affiliations

  • Published online on: December 1, 1999     https://doi.org/10.3892/ijo.15.6.1133
  • Pages: 1133-1139
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Abstract

Mutations of the p53 tumor suppressor gene have been found to be the single most frequent event in human cancers. In India and other southeast Asian countries tobacco chewing with betel quid was attributed to be the major factor in oral carcinogenesis. We have analyzed 72 untreated primary oral squamous cell carcinomas (SCCs) for mutations in the tumor suppressor gene p53 exons 4-9 by PCR-SSCP and DNA sequencing. Sequencing analysis revealed 16 missense mutations, one silent mutation in codon 307 and four A to G substitution polymorphism in codon 213. The incidence of p53 mutation was 21% (15 of 72) excluding the polymorphism and the silent mutation. Eight mutations were clustered in codons 266-282 of exon 8. Of the total mutation events 37.5% were G to A transitions and 31.3% were G to T transversions. These results indicate the possible involvement of tobacco derived nitrosamines and their adducts in the genesis of oral cancer among Indians.

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Dec 1999
Volume 15 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kannan K, Munirajan A, Krishnamurthy J, Bhuvarahamurthy V, Mohanprasad B, Panishankar K, Tsuchida N and Shanmugam G: Low incidence of p53 mutations in betel quid and tobacco chewing-associated oral squamous carcinoma from India.. Int J Oncol 15: 1133-1139, 1999
APA
Kannan, K., Munirajan, A., Krishnamurthy, J., Bhuvarahamurthy, V., Mohanprasad, B., Panishankar, K. ... Shanmugam, G. (1999). Low incidence of p53 mutations in betel quid and tobacco chewing-associated oral squamous carcinoma from India.. International Journal of Oncology, 15, 1133-1139. https://doi.org/10.3892/ijo.15.6.1133
MLA
Kannan, K., Munirajan, A., Krishnamurthy, J., Bhuvarahamurthy, V., Mohanprasad, B., Panishankar, K., Tsuchida, N., Shanmugam, G."Low incidence of p53 mutations in betel quid and tobacco chewing-associated oral squamous carcinoma from India.". International Journal of Oncology 15.6 (1999): 1133-1139.
Chicago
Kannan, K., Munirajan, A., Krishnamurthy, J., Bhuvarahamurthy, V., Mohanprasad, B., Panishankar, K., Tsuchida, N., Shanmugam, G."Low incidence of p53 mutations in betel quid and tobacco chewing-associated oral squamous carcinoma from India.". International Journal of Oncology 15, no. 6 (1999): 1133-1139. https://doi.org/10.3892/ijo.15.6.1133