Detection of ornithine decarboxylase mRNA in human breast cancer MCF-7 cells by in situ RT-PCR.
- L Clerico
- T Mancuso
- S Crovella
- A Amoroso
- M Morgutti
- E Demori
- S Parodi
Affiliations: Laboratory of Experimental Oncology, National Institute for Cancer Research, I-16132 Genova, Italy.
- Published online on: February 1, 2000 https://doi.org/10.3892/ijo.16.2.241
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
We have studied the expression of ornithine decarboxylase (ODC) mRNA by in situ hybridization and in situ RT-PCR in human breast cancer MCF-7 cell line. In situ RT-PCR demonstrated the overexpression of ODC mRNA, localized over the cytoplasm, while a very low signal was detected by in situ hybridization. Our findings indicate that in situ RT-PCR could represent a useful tool to study different levels of ODC expression in normal and tumor tissues. Since ODC expression is regulated by c-Myc oncoprotein, this model could be useful to monitor in vivo the effects of new anti-neoplastic molecules, specific inhibitors of c-Myc.