Detection of ornithine decarboxylase mRNA in human breast cancer MCF-7 cells by in situ RT-PCR.

  • Authors:
    • L Clerico
    • T Mancuso
    • S Crovella
    • A Amoroso
    • M Morgutti
    • E Demori
    • S Parodi
  • View Affiliations

  • Published online on: February 1, 2000     https://doi.org/10.3892/ijo.16.2.241
  • Pages: 241-245
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Abstract

We have studied the expression of ornithine decarboxylase (ODC) mRNA by in situ hybridization and in situ RT-PCR in human breast cancer MCF-7 cell line. In situ RT-PCR demonstrated the overexpression of ODC mRNA, localized over the cytoplasm, while a very low signal was detected by in situ hybridization. Our findings indicate that in situ RT-PCR could represent a useful tool to study different levels of ODC expression in normal and tumor tissues. Since ODC expression is regulated by c-Myc oncoprotein, this model could be useful to monitor in vivo the effects of new anti-neoplastic molecules, specific inhibitors of c-Myc.

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February 2000
Volume 16 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Clerico, L., Mancuso, T., Crovella, S., Amoroso, A., Morgutti, M., Demori, E., & Parodi, S. (2000). Detection of ornithine decarboxylase mRNA in human breast cancer MCF-7 cells by in situ RT-PCR.. International Journal of Oncology, 16, 241-245. https://doi.org/10.3892/ijo.16.2.241
MLA
Clerico, L., Mancuso, T., Crovella, S., Amoroso, A., Morgutti, M., Demori, E., Parodi, S."Detection of ornithine decarboxylase mRNA in human breast cancer MCF-7 cells by in situ RT-PCR.". International Journal of Oncology 16.2 (2000): 241-245.
Chicago
Clerico, L., Mancuso, T., Crovella, S., Amoroso, A., Morgutti, M., Demori, E., Parodi, S."Detection of ornithine decarboxylase mRNA in human breast cancer MCF-7 cells by in situ RT-PCR.". International Journal of Oncology 16, no. 2 (2000): 241-245. https://doi.org/10.3892/ijo.16.2.241