Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction.

  • Authors:
    • Y Miyake
    • Y Fujiwara
    • M Ohue
    • H Yamamoto
    • Y Sugita
    • N Tomita
    • M Sekimoto
    • H Shiozaki
    • M Monden
  • View Affiliations

  • Published online on: February 1, 2000     https://doi.org/10.3892/ijo.16.2.289
  • Pages: 289-382
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Abstract

The expression of carcinoembryonic antigen (CEA) mRNA was assessed in 102 lymph nodes (LNs) obtained from seven colorectal cancer patients by both the conventional non-quantitative RT-PCR and quantitative RT-PCR. The number of CEA-expressing cells was calculated compared with CEA-expressing MKN-45 cell line as a standard control. Using the quantitative RT-PCR, the relative number of CEA-expressing cells ranged between 1.3x103 and 5.7x106 in 16 histologically positive LNs and between 2.3x101 and 8.1x105 in 10 histologically negative and RT-PCR positive LNs. In both histologically and RT-PCR negative LNs, the relative cell number was <4.0x102. Our results demonstrated that quantifying the amount of metastasis might enhance the reliability of RT-PCR detection assay as a diagnostic tool for the detection of cancer micrometastases.

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February 2000
Volume 16 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Miyake, Y., Fujiwara, Y., Ohue, M., Yamamoto, H., Sugita, Y., Tomita, N. ... Monden, M. (2000). Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction.. International Journal of Oncology, 16, 289-382. https://doi.org/10.3892/ijo.16.2.289
MLA
Miyake, Y., Fujiwara, Y., Ohue, M., Yamamoto, H., Sugita, Y., Tomita, N., Sekimoto, M., Shiozaki, H., Monden, M."Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction.". International Journal of Oncology 16.2 (2000): 289-382.
Chicago
Miyake, Y., Fujiwara, Y., Ohue, M., Yamamoto, H., Sugita, Y., Tomita, N., Sekimoto, M., Shiozaki, H., Monden, M."Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction.". International Journal of Oncology 16, no. 2 (2000): 289-382. https://doi.org/10.3892/ijo.16.2.289