AMY-1 is a trigger for the erythrocyte differentiation of K562 cells.

  • Authors:
    • M Furusawa
    • T Onishi
    • T Taira
    • S M Iguchi-Ariga
    • H Ariga
  • View Affiliations

  • Published online on: February 1, 2000     https://doi.org/10.3892/ijo.16.2.339
  • Pages: 339-384
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Abstract

We have reported that a novel c-Myc binding protein, AMY-1, stimulated the transcription activity of c-Myc and was translocated from cytoplasm to nuclei in a c-Myc-dependent manner. Here, the role of AMY-1 in cell differentiation was examined. AMY-1 expression was up-regulated after differentiation induction of human K562 cells to erythrocyte cells by AraC, while c-Myc expression was rapidly down-regulated. K562 cell lines expressing exogenous AMY-1 were established, and these cells expressed a high level of epsilon-globin mRNA, a marker gene necessary for erythrocyte cell differentiation, without differentiation induction. The addition of AraC rapidly initiated differentiation in these cell lines, which continued to differentiate to erythrocyte cells possessing a high level of hemoglobin even after the decrease in AMY-1 expression. These results suggest that AMY-1 is a trigger for K562 cells to differentiate to erythrocyte cells and that AMY-1 may have a function independent of or different from c-Myc.

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February 2000
Volume 16 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Furusawa, M., Onishi, T., Taira, T., Iguchi-Ariga, S., & Ariga, H. (2000). AMY-1 is a trigger for the erythrocyte differentiation of K562 cells.. International Journal of Oncology, 16, 339-384. https://doi.org/10.3892/ijo.16.2.339
MLA
Furusawa, M., Onishi, T., Taira, T., Iguchi-Ariga, S., Ariga, H."AMY-1 is a trigger for the erythrocyte differentiation of K562 cells.". International Journal of Oncology 16.2 (2000): 339-384.
Chicago
Furusawa, M., Onishi, T., Taira, T., Iguchi-Ariga, S., Ariga, H."AMY-1 is a trigger for the erythrocyte differentiation of K562 cells.". International Journal of Oncology 16, no. 2 (2000): 339-384. https://doi.org/10.3892/ijo.16.2.339