Human colon cancer cells express multiple glycoprotein ligands for E-selectin.

  • Authors:
    • J Tomlinson
    • J L Wang
    • S H Barsky
    • M C Lee
    • J Bischoff
    • M Nguyen
  • View Affiliations

  • Published online on: February 1, 2000     https://doi.org/10.3892/ijo.16.2.347
  • Pages: 347-400
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The interaction between the colon tumor cell surface and the endothelial cell layer is an important component of tumor intravasation, extravasation, and metastasis. Multiple studies suggest that tumor cells may bind to E-selectin expressed on endothelial cells during these processes. To identify possible E-selectin ligands on tumor cells that may participate in this mechanism, we used E-selectin-Ig chimera affinity chromatography to isolate glycoproteins from the human colon cancer cell line Colo-205. Binding of these cells to E-selectin was specific, required the presence of calcium, and could be blocked by antibodies against E-selectin. We identified LAMP-1 (lysosomal membrane glycoprotein-1), LAMP-2, and two high molecular weight glycoproteins (>400 kDa and 300 kDa) as the main E-selectin ligands on Colo-205 cells. Treatment of the cells with N-glycanase and O-sialoglycoprotease abolished their binding to E-selectin. The high MW glycoproteins contained sialyl Lewis X and/or sialyl Lewis A glycoconjugates, and appeared to be either alternatively spliced or alternatively glycosylated forms of MUC-1 (mucin-1).

Related Articles

Journal Cover

February 2000
Volume 16 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Tomlinson, J., Wang, J., Barsky, S., Lee, M., Bischoff, J., & Nguyen, M. (2000). Human colon cancer cells express multiple glycoprotein ligands for E-selectin.. International Journal of Oncology, 16, 347-400. https://doi.org/10.3892/ijo.16.2.347
MLA
Tomlinson, J., Wang, J., Barsky, S., Lee, M., Bischoff, J., Nguyen, M."Human colon cancer cells express multiple glycoprotein ligands for E-selectin.". International Journal of Oncology 16.2 (2000): 347-400.
Chicago
Tomlinson, J., Wang, J., Barsky, S., Lee, M., Bischoff, J., Nguyen, M."Human colon cancer cells express multiple glycoprotein ligands for E-selectin.". International Journal of Oncology 16, no. 2 (2000): 347-400. https://doi.org/10.3892/ijo.16.2.347