Newcastle disease virus activates macrophages for anti-tumor activity.

  • Authors:
    • V Schirrmacher
    • L Bai
    • V Umansky
    • L Yu
    • Y Xing
    • Z Qian
  • View Affiliations

  • Published online on: February 1, 2000     https://doi.org/10.3892/ijo.16.2.363
  • Pages: 363-436
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Abstract

Newcastle Disease Virus (NDV), an agent with interesting immune stimulatory and anti-tumor activity, was investigated for its capacity to activate anti-tumor activity in murine macrophages in vitro and in vivo. Direct macrophage activation was seen under a variety of experimental conditions using two different strains of NDV, different sources of macrophages (spleen and peritoneum) and different strains of mice (DBA/2, C57BL/6, 615). Various macrophage enzymes (ADA, iNOS, lysozyme, acid phosphatase) became upregulated and anti-tumor effector molecules such as nitric oxide (NO) and TNF-alpha were found in the supernatant. NDV activated macrophages performed anti-tumor activity in vitro such as anti-tumor cytostasis and anti-tumor cytotoxicity. The cytotoxic anti-tumor activity was broad and active against all tumor lines tested including mammary carcinoma, lung carcinoma, mastocytoma and immune escape variants (lymphoma). Macrophage activation via BCG/LPS also caused a broad range anti-tumor cytotoxic activity while activation via mixed lymphocyte culture conditioned medium had restricted anti-tumor activity. Anti-tumor activity of NDV activated macrophages could be transfered in vivo. Transfer of macrophages which had not been appropriately activated exerted either no effect or a tumor growth augmenting effect. Repeated intravenous transfer of NDV activated macrophages exerted a significant suppressive effect on pulmonary metastases in a mammary carcinoma tumor model as well as in a lung carcinoma model. Taken together these results demonstrate that NDV can strongly activate macrophages to perform anti-tumor activities in vitro and in vivo.

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February 2000
Volume 16 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Schirrmacher, V., Bai, L., Umansky, V., Yu, L., Xing, Y., & Qian, Z. (2000). Newcastle disease virus activates macrophages for anti-tumor activity.. International Journal of Oncology, 16, 363-436. https://doi.org/10.3892/ijo.16.2.363
MLA
Schirrmacher, V., Bai, L., Umansky, V., Yu, L., Xing, Y., Qian, Z."Newcastle disease virus activates macrophages for anti-tumor activity.". International Journal of Oncology 16.2 (2000): 363-436.
Chicago
Schirrmacher, V., Bai, L., Umansky, V., Yu, L., Xing, Y., Qian, Z."Newcastle disease virus activates macrophages for anti-tumor activity.". International Journal of Oncology 16, no. 2 (2000): 363-436. https://doi.org/10.3892/ijo.16.2.363