The effect of p53-function on the sensitivity to paclitaxel with or without hyperthermia in human colorectal carcinoma cells.

van Bree,C; Savonije,J H; Franken,N A; Haveman,J; Bakker,P J

doi:10.3892/ijo.16.4.739

The effect of p53-function on the sensitivity to paclitaxel with or without hyperthermia in human colorectal carcinoma cells.

Authors:
- C van Bree
- J H Savonije
- N A Franken
- J Haveman
- P J Bakker
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Affiliations: Department of Radiotherapy, Academic Medical Center, University of Amsterdam, 1100 DE Amsterdam, The Netherlands.
Published online on: April 1, 2000 https://doi.org/10.3892/ijo.16.4.739
Pages: 739-783

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Abstract

The importance of p53-function for the sensitivity to paclitaxel with and without hyperthermia (HT) was studied in an isogenic cell line system. The inactivation of p53 decreased sensitivity to paclitaxel (1.1-2.5-fold), which correlated with a lower induction of apoptosis. The magnitude of the G2/M arrest after treatment with paclitaxel was similar in all cell lines. The cytotoxicity of paclitaxel was not enhanced by HT in either wild-type p53 or p53-inactivated cells. In conclusion, cellular sensitivity to paclitaxel depends on p53-function by its ability to induce apoptosis. Irrespective of the p53-function HT was not able to enhance the sensitivity to paclitaxel.