A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.

  • Authors:
    • H Sonobe
    • T Takeuchi
    • M Furihata
    • T Taguchi
    • A Kawai
    • Y Ohjimi
    • H Iwasaki
    • Y Kaneko
    • Y Ohtsuki
  • View Affiliations

  • Published online on: August 1, 2000     https://doi.org/10.3892/ijo.17.2.347
  • Pages: 347-399
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Abstract

Only a few human malignant peripheral nerve sheath tumour (MPNST)-cell lines have been reported, and their characteristics have not been fully established. In this study, we established a new human cell line, HS-Sch-2, from an MPNST of the ordinary type which arose in a 54-year-old woman without von Recklinghausen's disease. This cell line was characterized by chromosome analysis, immunohistochemistry, ultrastructural examination, and direct sequencing of the p53 gene. The HS-Sch-2 cells have grown for more than 48 months in vitro, and exhibited hypotriploid karyotypes with complex chromosome abnormalities lacking a specific pattern. Histological features of the heterotranplanted nude mouse tumours were essentially the same as those of the original MPNST, with positive reactions for S-100 protein and neuron-specific enolase but not for epithelial membrane antigen, fibronectin or CD34. Ultrastructural examination in vivo revealed intricate interdigitation of long cytoplasmic processes and basal lamina-like structures. In addition, direct sequencing of the p53 gene detected a point mutation from CGT to CAT at codon 273 in exon 8. This HS-Sch-2 cell line, which exhibits distinctive morphological characteristics of MPNST and a p53 point mutation, will be useful for biological and pathological investigations of MPNST.

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August 2000
Volume 17 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Sonobe, H., Takeuchi, T., Furihata, M., Taguchi, T., Kawai, A., Ohjimi, Y. ... Ohtsuki, Y. (2000). A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.. International Journal of Oncology, 17, 347-399. https://doi.org/10.3892/ijo.17.2.347
MLA
Sonobe, H., Takeuchi, T., Furihata, M., Taguchi, T., Kawai, A., Ohjimi, Y., Iwasaki, H., Kaneko, Y., Ohtsuki, Y."A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.". International Journal of Oncology 17.2 (2000): 347-399.
Chicago
Sonobe, H., Takeuchi, T., Furihata, M., Taguchi, T., Kawai, A., Ohjimi, Y., Iwasaki, H., Kaneko, Y., Ohtsuki, Y."A new human malignant peripheral nerve sheath tumour-cell line, HS-sch-2, harbouring p53 point mutation.". International Journal of Oncology 17, no. 2 (2000): 347-399. https://doi.org/10.3892/ijo.17.2.347