CDDP induces p53-dependent apoptosis in tongue cancer cells.

  • Authors:
    • H Kanata
    • K Yane
    • I Ota
    • H Miyahara
    • T Matsunaga
    • A Takahashi
    • K Ohnishi
    • T Ohnishi
    • H Hosoi
  • View Affiliations

  • Published online on: September 1, 2000     https://doi.org/10.3892/ijo.17.3.513
  • Pages: 513-520
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Abstract

We have investigated the CDDP sensitivities of two tongue cancer cell lines with differing p53 genetic status, one with wild-type p53 (SAS) and the other with mutant-type p53 (HSC-4). SAS was about 2 times more sensitive at the D10 dose and demonstrated increased p53 and Bax protein levels at 10 h after CDDP treatment on Western blot analysis. On the other hand, overexpression of p53 in HSC-4 was observed without CDDP treatment and no elevation of Bax could be detected. Apoptosis was observed after CDDP treatment in SAS but not in HSC-4 by Hoechst 33342-staining and electrophoresis methods. These findings indicate that p53 plays an important role in apoptosis as a positive regulator of Bax expression. It is suggested that p53 status may have predictive potential with regard to response to CDDP therapy.

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September 2000
Volume 17 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kanata, H., Yane, K., Ota, I., Miyahara, H., Matsunaga, T., Takahashi, A. ... Hosoi, H. (2000). CDDP induces p53-dependent apoptosis in tongue cancer cells.. International Journal of Oncology, 17, 513-520. https://doi.org/10.3892/ijo.17.3.513
MLA
Kanata, H., Yane, K., Ota, I., Miyahara, H., Matsunaga, T., Takahashi, A., Ohnishi, K., Ohnishi, T., Hosoi, H."CDDP induces p53-dependent apoptosis in tongue cancer cells.". International Journal of Oncology 17.3 (2000): 513-520.
Chicago
Kanata, H., Yane, K., Ota, I., Miyahara, H., Matsunaga, T., Takahashi, A., Ohnishi, K., Ohnishi, T., Hosoi, H."CDDP induces p53-dependent apoptosis in tongue cancer cells.". International Journal of Oncology 17, no. 3 (2000): 513-520. https://doi.org/10.3892/ijo.17.3.513